Overview

  • Product name
  • Description
    Mouse monoclonal to MTHFD2
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 16-345 of Human MTHFD2

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab176016 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab56772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Sequence similarities
    Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
  • Developmental stage
    Expressed only in developing normal tissues.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial antibody
    • Descriptions antibody
    • Methenyltetrahydrofolate cyclohydrolase antibody
    • methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody
    • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 antibody
    • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) antibody
    • methylenetetrahydrofolate dehydrogenase 2 antibody
    • MGC82516 antibody
    • MTDC_HUMAN antibody
    • MTHFD2 antibody
    • mthfd2 methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody
    • NAD-dependent methylene tetrahydrofolate dehydrogenase antibody
    • NAD-dependent methylenetetrahydrofolate dehydrogenase antibody
    • NMDMC antibody
    see all

Images

  • MTHFD2 antibody (ab56772) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma tissue.


  • Predicted band size : 37 kDa
    MTHFD2 antibody (ab56772) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
  • Overlay histogram showing HepG2 cells stained with ab56772 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56772, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Gustafsson Sheppard N  et al. The folate-coupled enzyme MTHFD2 is a nuclear protein and promotes cell proliferation. Sci Rep 5:15029 (2015). WB . Read more (PubMed: 26461067) »
  • Nilsson R  et al. Metabolic enzyme expression highlights a key role for MTHFD2 and the mitochondrial folate pathway in cancer. Nat Commun 5:3128 (2014). WB ; Human . Read more (PubMed: 24451681) »

See all 2 Publications for this product

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