The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/128000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Isoform 1, isoform 4 and isoform 5 are expressed in brain and placenta.