Overview

  • Product name
  • Description
    Goat polyclonal to MTHFS
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    KRCLQHQEVKPYT

    , corresponding to internal sequence amino acids 158-170 of human MTHFS (NP_006432.1)

  • Positive control
    • Human liver lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab82153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 23 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.
    • Sequence similarities
      Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 10-methenyl-tetrahydrofolate synthetase antibody
      • 5 10 methenyl tetrahydrofolate synthetase antibody
      • 5 antibody
      • 5 formyltetrahydrofolate cyclo ligase antibody
      • 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) antibody
      • 5-formyltetrahydrofolate cyclo-ligase antibody
      • FLJ30410 antibody
      • HsT19268 antibody
      • Methenyl THF synthetase antibody
      • Methenyl-THF synthetase antibody
      • MTHFS antibody
      • MTHFS_HUMAN antibody
      see all

    Images

    • Anti-MTHFS antibody (ab82153) at 0.5 µg/ml + human liver lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 23 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 hour

    References

    ab82153 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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