Overview

  • Product nameAnti-MTMR1 antibody
    See all MTMR1 primary antibodies
  • Description
    Rabbit polyclonal to MTMR1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: FLCNCEQQRF KEDVYTKTIS LWSYINSQLD EFSNPFFVNY ENHVLYPVAS, corresponding to C terminal amino acids 541-590 of Human MTMR1

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately >70 kDa (predicted molecular weight: 75 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceMTMR1 is a member of the myotubularin related family of proteins. Members of this family contain the consensus sequence for the active site of protein tyrosine phosphatases. The MTMR1 gene shares structural similarities with the MTM1 gene, and is also located in the same chromosomal region. Myotubularin related proteins are a large subfamily of protein tyrosine phosphatases (PTPs) that dephosphorylate D3 phosphorylated inositol lipids. MTMR1 is a lipid phosphatase that acts on phosphatidylinositol 3-phosphate and phosphatidylinositol (3,5)-bisphosphate.
  • Cellular localizationPlasma Membrane; Peripheral membrane protein, cytoplasmic side.
  • Database links
  • Alternative names
    • MTMR 1 antibody
    • Myotubularin related 1 antibody
    • Myotubularin related protein 1 antibody

Anti-MTMR1 antibody images

  • Anti-MTMR1 antibody (ab66515) at 1.25 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 75 kDa
    Observed band size : >70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >>70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MTMR1 antibody (ab66515)

ab66515 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"