Overview

  • Product nameAnti-MTMR1 antibody
    See all MTMR1 primary antibodies
  • Description
    Mouse monoclonal to MTMR1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: SRQPSVETLD SPTGSHVEWC KQLIAATISS QISGSVTSEN VSRDYKALRD GNKLAQMEEA PLFPGESIKA IVKD, corresponding to amino acids 39-112 of human MTMR1 (NP_003819) with a 26kDa tag

  • Positive control
    • MTMR1 transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight for the full length protein: 75 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMTMR1 is a member of the myotubularin related family of proteins. Members of this family contain the consensus sequence for the active site of protein tyrosine phosphatases. The MTMR1 gene shares structural similarities with the MTM1 gene, and is also located in the same chromosomal region. Myotubularin related proteins are a large subfamily of protein tyrosine phosphatases (PTPs) that dephosphorylate D3 phosphorylated inositol lipids. MTMR1 is a lipid phosphatase that acts on phosphatidylinositol 3-phosphate and phosphatidylinositol (3,5)-bisphosphate.
    • Cellular localizationPlasma Membrane; Peripheral membrane protein, cytoplasmic side.
    • Database links
    • Alternative names
      • MTMR 1 antibody
      • Myotubularin related 1 antibody
      • Myotubularin related protein 1 antibody

    Anti-MTMR1 antibody images

    • Anti-MTMR1 antibody (ab76883) at 5 µg/ml + immunogen (recombinant tagged fragment) at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 75 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-MTMR1 antibody (ab76883) at 5 µg/ml

      Lane 1 : MTMR1 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 75 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    References for Anti-MTMR1 antibody (ab76883)

    ab76883 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76883.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"