Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mTOR antibody

Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Testis)
Specification
Testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric aicd
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/3000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
I am not familiar with the distribution pattern of this protein so I cannot confirm that the positivity is specific.
It gives a particular pattern of positivity in testis, however ( coloured arrowheads indicate this): positive nuclei ( red) appear to be confined to the stem cell layer of the seminiferous tubules whereas maturing cells appear to have light cytoplasmic positivity. In all tubes there is a lumenal cytoplasmic positivity that varies in intensity ( green) between tubules.
Leydig cells are also positive ( yellow).
Spaces around the tubes are artefactual.
Username

Mr. Carl Hobbs

Verified customer

Submitted Jul 06 2011

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