Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mTOR antibody

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Testis)
Specification Testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric aicd
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution 1/3000
Incubation time 2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide

Secondary antibody

Name Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution 1/200

Additional data

Additional Notes I am not familiar with the distribution pattern of this protein so I cannot confirm that the positivity is specific.
It gives a particular pattern of positivity in testis, however ( coloured arrowheads indicate this): positive nuclei ( red) appear to be confined to the stem cell layer of the seminiferous tubules whereas maturing cells appear to have light cytoplasmic positivity. In all tubes there is a lumenal cytoplasmic positivity that varies in intensity ( green) between tubules.
Leydig cells are also positive ( yellow).
Spaces around the tubes are artefactual.

Mr. Carl Hobbs

Verified customer

Submitted Jul 06 2011