Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mTOR antibody

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Testis)
Specification Testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution 1/2000
Incubation time 2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide

Secondary antibody

Name Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution 1/200

Additional data

Additional Notes I am not familiar with the distribution pattern of this protein.
Results on mouse and rat testis appear very similar.
Positive components in the submitted image are indicated by coloured arrowheads:
red for densely positive nuclei that appear to be confined to the stem cell layer ( immediately above, some spermatogonia exhibit a nuclear speckling)
Yellow indicates a moderate cytoplasmic positivity, more evident in some seminiferous tubules (ST).
Green for a strong apparently cytoplasmic positivity at the lumenal surface of a proportion of ST. To me, this does not appear to be spermatozoa positivity spermatids?Sertoli cells?

Mr. Carl Hobbs

Verified customer

Submitted Jul 06 2011