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A synthetic peptide corresponding to residues near the C-term of PI3K/PI4K domain of human mTOR/FRAP was used as an immunogen
Produced under U.S. Patent No. 5,675,063.
This product is available conjugated to DyLight® 488 see ab139837.
Our Abpromise guarantee covers the use of ab32028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration. PubMed: 20233713|
|WB||1/1000 - 1/2000. Detects a band of approximately >250 kDa (predicted molecular weight: 289 kDa).Can be blocked with mTOR peptide (ab193663).|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/50 - 1/80.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"