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A synthetic peptide corresponding to residues near the C-term of PI3K/PI4K domain of human mTOR/FRAP was used as an immunogen
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Our Abpromise guarantee covers the use of ab32028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 20233713|
|WB||1/1000 - 1/2000. Detects a band of approximately 250 kDa (predicted molecular weight: 289 kDa).Can be blocked with mTOR peptide (ab193663).|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/50 - 1/80.
Ab172730-Rabbit monoclonal IgG,is suitable for use as an isotype control with this antiobody.
|IHC-P||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"