Overview

  • Product nameAnti-MTR antibody
    See all MTR primary antibodies
  • Description
    Goat polyclonal to MTR
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig
  • Immunogen

    Synthetic peptide: VEKWLGPILGYDTD, corresponding to C terminal amino acids 1252-1265 of Human MTR.

  • Positive control
    • HeLa and Jurkat cell lysates
  • General notes


    This enzyme catalyzes the final step in methionine biosynthesis. MTR mutations have been identified as an underlying cause of methylcobalamin deficiency complementation group G. Principle Names - MTR; 5-methyltetrahydrofolate-homocysteine methyltransferase methionine synthase

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).Can be blocked with Human MTR peptide (ab23154).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceMTR encodes the enzyme 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • 5-methyltetrahydrofolate homocysteine methyltransferase antibody
    • 5-methyltetrahydrofolate-homocysteine methyltransferase antibody
    • cblG antibody
    • Methionine synthase antibody
    • methioninesynthase antibody
    • MS antibody
    • MTR antibody
    • MTR1 antibody
    • PRP20 antibody
    • SRM1 antibody
    • Vitamin-B12 dependent methionine synthase antibody
    see all

Anti-MTR antibody images

  • 9209 (3.8µg/ml) staining of paraffin embedded Human Adrenal Gland following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows cytoplasm staining in the cortex.



  • Predicted band size : 150 kDa

    Goat polyclonal to MTR (2µg/ml) staining of Hela lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.

References for Anti-MTR antibody (ab9209)

This product has been referenced in:
  • Fofou-Caillierez MB  et al. Interaction between methionine synthase isoforms and MMACHC: characterization in cblG-variant, cblG and cblC inherited causes of megaloblastic anaemia. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23825108) »
  • Zhang Y  et al. Homocysteine homeostasis and betaine-homocysteine S-methyltransferase expression in the brain of hibernating bats. PLoS One 8:e85632 (2013). WB . Read more (PubMed: 24376891) »

See all 2 Publications for this product

Product Wall

The band is either albumin or a degredation product containing the C-terminal (since the antibody is raised against a sequence from the c-terminal)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"