Overview

  • Product name
    Anti-mtRNA polymerase antibody
    See all mtRNA polymerase primary antibodies
  • Description
    Rabbit polyclonal to mtRNA polymerase
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity

    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1200 to the C-terminus of Human mtRNA polymerase.

    (Peptide available as ab32987.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates; HeLa, HEK293 and Caco2.

Properties

Applications

Our Abpromise guarantee covers the use of ab32988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 139 kDa).

Target

  • Function
    DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.
  • Sequence similarities
    Belongs to the phage and mitochondrial RNA polymerase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • APOLMT antibody
    • DNA-directed RNA polymerase antibody
    • DNA-directed RNA polymerase mitochondrial antibody
    • h-mtRPOL antibody
    • mitochondrial antibody
    • MTRNAP antibody
    • MtRPOL antibody
    • POLRMT antibody
    • polymerase (RNA) mitochondrial (DNA directed) antibody
    • polymerase, RNA, mitochondrial antibody
    • RPOM antibody
    • RPOM_HUMAN antibody
    see all

Anti-mtRNA polymerase antibody images

  • All lanes : Anti-mtRNA polymerase antibody (ab32988) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 139 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 160 kDa,45 kDa,65 kDa. We are unsure as to the identity of these extra bands.

References for Anti-mtRNA polymerase antibody (ab32988)

This product has been referenced in:
  • Nouws J  et al. Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import. J Biol Chem 291:989-97 (2016). Read more (PubMed: 26586915) »
  • Harmel J  et al. The leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) does not activate transcription in mammalian mitochondria. J Biol Chem 288:15510-9 (2013). WB ; Mouse . Read more (PubMed: 23599432) »

See all 4 Publications for this product

Product Wall

Vielen Dank für Ihren Anruf vom letzten Freitag.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten.

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Read More

Thank you for contacting us.

As discussed please remove the precipitates by centrifuging the vial and use it in western blot. If the antibody fails please do not hesitate to contact me for replacement.

Have a nice day!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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