Anti-mtTFA antibody [18G102B2E11] (ab119684)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab119684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionBinds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
  • Sequence similaritiesContains 2 HMG box DNA-binding domains.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • anscription factor 6-like 1 antibody
    • Mitochondrial transcription factor 1 antibody
    • mitochondrial transcription factor A antibody
    • MtTF1 antibody
    • mtTFA antibody
    • TCF 6 antibody
    • TCF-6 antibody
    • TCF6 antibody
    • TCF6L1 antibody
    • TCF6L2 antibody
    • TCF6L3 antibody
    • TFAM antibody
    • TFAM_HUMAN antibody
    • Transcription factor 6 antibody
    • Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
    • Transcription factor 6 like 2 antibody
    • Transcription factor 6-like 2 antibody
    • transcription factor 6-like 3 antibody
    • Transcription factor A, mitochondrial antibody
    • Transcription factor A, mitochondrial antibody
    • Transcription factor A, mitochondrial precursor antibody
    see all

Anti-mtTFA antibody [18G102B2E11] images

  • Immunocytochemistry image of ab119684 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (18G102B2E11, 1µg/ml) for 2h at room temperature. The secondary antibody was (green) 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). mtTFA localizes to the mitochondria.
  • HeLa cells were stained with 2 µg/mL mtTFA antibody (ab119684) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • All lanes : Anti-mtTFA antibody [18G102B2E11] (ab119684) at 1 µg/ml

    Lane 1 : recombinant mtTFA (ab103784) at 0.025 µg
    Lane 2 : HeLa whole cell lysate at 30 µg

    Secondary
    HRP-conjugated Goat polyclonal to mouse IgG at 1/5000 dilution

    Predicted band size : 29 kDa
  • IHC image of mtTFA staining in human liver HCC formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119684, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-mtTFA antibody [18G102B2E11] (ab119684)

This product has been referenced in:
  • Lopes Costa A  et al. Beneficial effects of resveratrol on respiratory chain defects in patients' fibroblasts involve estrogen receptor and estrogen-related receptor alpha signaling. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 24365713) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Muscle)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 15 µg
Specification Muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 10 2015


Upon reviewing your protocol, it is troubling to me that all 4 of these antibodies are not working. While it is conceivable that all four antibodies are not performing properly, typically when multiple antibodies are not working it is an issue wit...

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Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolée que vous avez des problèmes avec l'interprétation de deux de nos anticorps.

Comme expliqué aupara...

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Je suis désolée d'apprendre que ces anticorps ne vous donnent pas de résultats satisfaisants en Western blot - c.à.d. une bande à environ 42kD pour SURF1 et à environ 24kD pour mtTFA).
Nous n'avons pas eu des autres retours négatives et n'avons pa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"