Overview

  • Product nameAnti-mtTFA antibody
    See all mtTFA primary antibodies
  • Description
    Rabbit polyclonal to mtTFA
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human mtTFA.

    (Peptide available as ab47516.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa, Jurkat, A431. This antibody gave a positive signal in the following tissue lysates: Skeletal Muscle (Human) - adult normal tissue, Heart (Human) - adult normal tissue, Brown adipose (Mouse), Brown adipose (Rat), Heart (Mouse), Heart (Rat).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 29 kDa).Can be blocked with Human mtTFA peptide (ab47516).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionBinds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
  • Sequence similaritiesContains 2 HMG box DNA-binding domains.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • anscription factor 6-like 1 antibody
    • Mitochondrial transcription factor 1 antibody
    • mitochondrial transcription factor A antibody
    • MtTF1 antibody
    • mtTFA antibody
    • TCF 6 antibody
    • TCF-6 antibody
    • TCF6 antibody
    • TCF6L1 antibody
    • TCF6L2 antibody
    • TCF6L3 antibody
    • TFAM antibody
    • TFAM_HUMAN antibody
    • Transcription factor 6 antibody
    • Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
    • Transcription factor 6 like 2 antibody
    • Transcription factor 6-like 2 antibody
    • transcription factor 6-like 3 antibody
    • Transcription factor A, mitochondrial antibody
    • Transcription factor A, mitochondrial antibody
    • Transcription factor A, mitochondrial precursor antibody
    see all

Anti-mtTFA antibody images

  • All lanes : Anti-mtTFA antibody (ab47517) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Brown adipose (Mouse) Tissue Lysate
    Lane 4 : Brown adipose (Rat) Tissue Lysate
    Lane 5 : Heart (Mouse) Tissue Lysate
    Lane 6 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Additional bands at : 100 kDa,18 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab47517 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • ICC/IF image of ab47517 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab47517, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • IHC-P image of mtTFA staining using ab47517 in kidney (upper) and Colon (lower) sections. The tissue was fixed using formaldehyde. Heat mediated epitope retrieval was carried out using citric acid (pH 6). The sections were blocked uwing 1% BSA for 10 mins at 21°C.

    See Abreview

  • All lanes : Anti-mtTFA antibody (ab47517) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-mtTFA antibody (ab47517) at 2 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human mtTFA peptide (ab47516) at 2 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 24,26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

References for Anti-mtTFA antibody (ab47517)

This product has been referenced in:
  • Schiffer TA  et al. Dynamic regulation of metabolic efficiency explains tolerance to acute hypoxia in humans. FASEB J 28:4303-11 (2014). Read more (PubMed: 24970395) »
  • Sage JM  et al. Discovery of a novel function for human Rad51: maintenance of the mitochondrial genome. J Biol Chem 285:18984-90 (2010). WB ; Human . Read more (PubMed: 20413593) »

See all 4 Publications for this product

Product Wall

Thank you for confirming the results of the customer. Unfortunately, Anja is not in the office at present and is therefore unable to help resolve this case but I will try my best in her place.

I think the results look much improved with the b...

Read More

Thank you for contacting us.

I am sorry to hear you have been experiencing problems with a vial of anti-mtTFA antibody ab47517.

We have received no complaints about this product therefore I am concerned it is not working for you. ...

Read More

Thank you for contacting us. My colleague "Ariana" has already sent her reply so I am closing this case. The antibodies are fine for 1 week at room temperature however in your case if the products fail to perform I would suggest getting refund ...

Read More

Thank you for contacting us. In order to help you with your question could you provide the following - Order number of purchase - for how long the power cut happened? -Were these products at room temperature when the electricity switc...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon and Kidney)
Specification Colon and Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 12.5 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jun 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK 293 cells)
Loading amount 20 µg
Specification HEK 293 cells
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Sarah Ronnebaum

Verified customer

Submitted May 19 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"