The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimize staining. Microwave method recommended.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionBinds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
ab89818 at 10µg/ml staining mtTFA in PFA-fixed, permeabilized HeLa cells by immunofluorescence.
References for Anti-mtTFA antibody (ab89818)
This product has been referenced in:
Riley LG et al. Phenotypic variability and identification of novel YARS2 mutations in YARS2 mitochondrial myopathy, lactic acidosis and sideroblastic anaemia. Orphanet J Rare Dis8:193 (2013).
Read more (PubMed: 24344687) »
Berquist BR et al. Human Cockayne syndrome B protein reciprocally communicates with mitochondrial proteins and promotes transcriptional elongation. Nucleic Acids Res40:8392-405 (2012).
Read more (PubMed: 22743267) »