Overview

  • Product nameAnti-mtTFA antibody
    See all mtTFA primary antibodies
  • Description
    Mouse polyclonal to mtTFA
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1 - 246 of Human mtTFA (NP_003192.1)

  • Positive control
    • WB: kidney lysate. IHC: small intestine tissue. ICC/IF: HeLa cells.

Associated products

Applications

Our Abpromise guarantee covers the use of ab89818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimize staining. Microwave method recommended.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
    • Sequence similaritiesContains 2 HMG box DNA-binding domains.
    • Cellular localizationMitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • anscription factor 6-like 1 antibody
      • Mitochondrial transcription factor 1 antibody
      • mitochondrial transcription factor A antibody
      • MtTF1 antibody
      • mtTFA antibody
      • TCF 6 antibody
      • TCF-6 antibody
      • TCF6 antibody
      • TCF6L1 antibody
      • TCF6L2 antibody
      • TCF6L3 antibody
      • TFAM antibody
      • TFAM_HUMAN antibody
      • Transcription factor 6 antibody
      • Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
      • Transcription factor 6 like 2 antibody
      • Transcription factor 6-like 2 antibody
      • transcription factor 6-like 3 antibody
      • Transcription factor A, mitochondrial antibody
      • Transcription factor A, mitochondrial antibody
      • Transcription factor A, mitochondrial precursor antibody
      see all

    Anti-mtTFA antibody images

    • Anti-mtTFA antibody (ab89818) at 1 µg/ml + Human kidney lysate at 50 µg

      Predicted band size : 29 kDa
    • All lanes : Anti-mtTFA antibody (ab89818) at 1 µg/ml

      Lane 1 : mtTFA transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 29 kDa
      Observed band size : 29 kDa
      Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
    • ab89818 at 3µg/ml staining mtTFA in formalin-fixed, paraffin-embedded Human small intestine tissue by immunohistochemistry.
    • ab89818 at 10µg/ml staining mtTFA in PFA-fixed, permeabilized HeLa cells by immunofluorescence.

    References for Anti-mtTFA antibody (ab89818)

    This product has been referenced in:
    • Riley LG  et al. Phenotypic variability and identification of novel YARS2 mutations in YARS2 mitochondrial myopathy, lactic acidosis and sideroblastic anaemia. Orphanet J Rare Dis 8:193 (2013). WB . Read more (PubMed: 24344687) »
    • Berquist BR  et al. Human Cockayne syndrome B protein reciprocally communicates with mitochondrial proteins and promotes transcriptional elongation. Nucleic Acids Res 40:8392-405 (2012). Read more (PubMed: 22743267) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"