The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Multiple alternatively spliced transcript variants encoding different isoforms have been found for the MTUS1 (Mitochondrial tumor suppressor 1) gene. One of the transcript variants has been shown to encode a mitochondrial protein that acts as a tumor suppressor and cooperates with the angiotension II (AT2) receptor (AGTR2) to inhibit ERK2 activation and cell proliferation. MTUS1 polypeptides are identical in their carboxy-terminal region carrying four coiled-coil domains. In their amino-terminal portion, MTUS1 polypeptides exhibit distinct motifs for localisation in the cytosol, nucleus or cell membrane, suggesting that MTUS1 gene products may be involved in a variety of intracellular functions in an AT2-dependent and independent manner.
Cell Membrane, Golgi Apparatus, Mitochondrial and Nuclear. Note=In neurons, translocates into the nucleus after treatment with angiotensin-II.