The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml.
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceMultiple alternatively spliced transcript variants encoding different isoforms have been found for the MTUS1 (Mitochondrial tumor suppressor 1) gene. One of the transcript variants has been shown to encode a mitochondrial protein that acts as a tumor suppressor and cooperates with the angiotension II (AT2) receptor (AGTR2) to inhibit ERK2 activation and cell proliferation. MTUS1 polypeptides are identical in their carboxy-terminal region carrying four coiled-coil domains. In their amino-terminal portion, MTUS1 polypeptides exhibit distinct motifs for localisation in the cytosol, nucleus or cell membrane, suggesting that MTUS1 gene products may be involved in a variety of intracellular functions in an AT2-dependent and independent manner.
Cellular localizationCell Membrane, Golgi Apparatus, Mitochondrial and Nuclear. Note=In neurons, translocates into the nucleus after treatment with angiotensin-II.
Overlay histogram showing HeLa cells stained with ab57477 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57477, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - MTUS1 antibody (ab57477)
MTUS1 antibody (ab57477) at 1ug/lane + K-562 cell lysate at 25ug/lane.
References for Anti-MTUS1 antibody (ab57477)
has not yet been referenced specifically in any publications.
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