Overview

  • Product nameAnti-MTX2 antibody
    See all MTX2 primary antibodies
  • Description
    Rabbit polyclonal to MTX2
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (YIAAEPWPEN ATLYQQLKGE QILLSDNAAS LAVQAFLQMC NLPIKVVCRA) of human MTX2 (NP_001006636).

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:12500.

IHC-P 1/100.

Target

Anti-MTX2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling MTX2 with ab83015 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - MTX2. Right - Merge.


  • Predicted band size : 29 kDa
    Western blot analysis of Jurkat cell lysate labeling MTX2 with ab83015 at 1.0µg/ml.

References for Anti-MTX2 antibody (ab83015)

ab83015 has not yet been referenced specifically in any publications.

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