Overview

  • Product name
    Anti-mu Crystallin antibody
    See all mu Crystallin primary antibodies
  • Description
    Mouse monoclonal to mu Crystallin
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human mu Crystallin aa 215-314.
    Sequence:

    EWVKPGAHINAVGASRPDWRELDDELMKEAVLYVDSQEAALKESGDVLLS GAEIFAELGEVIKGVKPAHCEKTTVFKSLGMAVEDTVAAKLIYDSWSSGK

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab54669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 34 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Binds thyroid hormone. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors.
  • Tissue specificity
    Expressed in neural tissue, muscle and kidney.
  • Sequence similarities
    Belongs to the ornithine cyclodeaminase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CRYM antibody
    • CRYM_HUMAN antibody
    • Crystallin mu antibody
    • DFNA 40 antibody
    • DFNA40 antibody
    • Ketimine reductase antibody
    • Mu crystallin homolog antibody
    • Mu-crystallin homolog antibody
    • NADP regulated thyroid hormone binding protein antibody
    • NADP-regulated thyroid-hormone-binding protein antibody
    • OTTHUMP00000115878 antibody
    • THBP antibody
    • Thiomorpholine carboxylate dehydrogenase antibody
    see all

Images



  • Predicted band size : 34 kDa
    mu Crystallin antibody (ab54669) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
  • ICC/IF image of ab54669 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54669, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • mu Crystallin antibody (ab54669) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human skeletal muscle.
  • Overlay histogram showing SH-SY5Y cells stained with ab54669 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54669, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Zuccotti A  et al. The transcription factor Fezf2 directs the differentiation of neural stem cells in the subventricular zone toward a cortical phenotype. Proc Natl Acad Sci U S A 111:10726-31 (2014). Read more (PubMed: 25002477) »
  • Davidson B  et al. Gene expression signatures of primary and metastatic uterine leiomyosarcoma. Hum Pathol 45:691-700 (2014). IHC . Read more (PubMed: 24485798) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse retina whole cell lysate)
Loading amount
30 µg
Specification
mouse retina whole cell lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Nov 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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