Fluorogenic substrate of β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase). Used for characterizing novel β-glucosidases. Also used in assays to evaluate deficiency in β-glucocerebrosidase activity related to Gaucher disease. Hydrolysis releases the fluorescent product 4-MU.
Fluorescence: λex 365 nm; λem 445 nm in 0.1 M sodium acetate pH 5.0 (after cleavage by β-glucosidase).
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Hernández-Guzmán A et al. Purification and characterization of an extracellular ß-glucosidase from Sporothrix schenckii. FEBS Open Bio6:1067-1077 (2016).
Read more (PubMed: 27833847) »
Shanmuganathan M & Britz-McKibbin P Inhibitor screening of pharmacological chaperones for lysosomal ß-glucocerebrosidase by capillary electrophoresis. Anal Bioanal Chem399:2843-53 (2011).
Read more (PubMed: 21286689) »