Overview

  • Product name
    Anti-Mu Opioid Receptor antibody
    See all Mu Opioid Receptor primary antibodies
  • Description
    Rabbit polyclonal to Mu Opioid Receptor
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Fr, ICC, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human
    Predicted to work with: Cow, Pig, Non human primates, Macaque monkey
  • Immunogen

    Synthetic peptide:

    NHQLENLEAETAPLP

    , corresponding to C terminal amino acids 384-398 of Rat Mu Opioid Receptor 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab10275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/2000.
WB 1/500 - 1/1000. Detects a band of approximately 49-65 kDa (predicted molecular weight: 45 kDa).Can be blocked with Rat Mu Opioid Receptor peptide (ab46988).

Approximately 49 -65 kDa depending on the tissue lysate. Can be blocked with ab46988.

IHC-Fr 1/100 - 1/2000. We recommend incubating for 18-24 hours at 4C.
ICC 1/100 - 1/2000.
IHC-FoFr 1/100 - 1/2000. We recommend incubating for 18-24 hours at 4C.

Target

Images

  • Ab10275 (1:2500) staining of Mu opioid receptor by IHC.
  • ICC/IF image of ab10275 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10275, 1 in 200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of rat brain tissue, staining Mu Opioid Receptor with ab10275.

    Tissue was blocked with 5% normal donkey serum and 0.4% Triton X-100 for 1 hour at room temperature. Sections were incubated with primary antibody (1/2000) for 24 hours at 4°C. An AlexaFluor®594-conjugated donkey anti-rabbit IgG (1/250) was used as the secondary antibody.
  • Mu Opioid Receptor (ab10275) staining of Rat DRG (dilution: 1:100) incubation at 4 °C overnight. Secondary antibody is anti-Rabbit Rhodamine Red (dilution:1:200) incubation at room temperature for 1 hour.

References

This product has been referenced in:
  • Song C & Xue L Roles of the µ-opioid receptor and its related signaling pathways in the pathogenesis of premenstrual syndrome liver-qi stagnation. Exp Ther Med 13:3130-3136 (2017). Read more (PubMed: 28587388) »
  • Yang Z  et al. Reverse of Acute and Chronic Morphine Tolerance by Lithocholic Acid via Down-Regulating UGT2B7. Front Pharmacol 7:404 (2016). WB ; Mouse . Read more (PubMed: 27847477) »

See all 23 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse, whole brain sections)
Antigen retrieval step
None
Permeabilization
Yes - 10% Tween-20
Specification
Mouse, whole brain sections
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - other (Gross brain dissected tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Gross brain dissected tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 26 2016

Gracias por contactarnos.
Después de consultar este caso con mis compañeros del laboratorio, acerca de los siguientes anticuerpos contra Mu Opioid Receptor validados en tejidos de rata: ab10275, ab17934, ab64746, ab128013 y ab134054, me temo que n...

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I am sorry to hear that the free of charge replacement has not improved the results on this occasion. Due to your valuable information this antibody will now be further investiga...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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I am sorry to confirm that we don't have any different lots in stock at the moment. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from our Anti-Mu Opioid Receptor antibody (ab10275).

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the origi...

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Thanks for your enquiry.

ab10275 is a whole antiserum product and the exact concentration of the anti-Mu opioid receptor in the serum has not been determined. In general, however, specific antibody concentration inproducts of this type is typ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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