• Product nameAnti-Mu Opioid Receptor antibody
    See all Mu Opioid Receptor primary antibodies
  • Description
    Rabbit polyclonal to Mu Opioid Receptor
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Fr, ICC, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human
    Predicted to work with: Cow, Pig, Non Human Primates, Macaque monkey
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 384-398 of Rat Mu Opioid Receptor 1.



Our Abpromise guarantee covers the use of ab10275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/2000.
WB 1/500 - 1/1000. Detects a band of approximately 49-65 kDa (predicted molecular weight: 45 kDa).Can be blocked with Rat Mu Opioid Receptor peptide (ab46988).

Approximately 49 -65 kDa depending on the tissue lysate. Can be blocked with ab46988.

IHC-Fr 1/100 - 1/2000. We recommend incubating for 18-24 hours at 4C.
ICC 1/100 - 1/2000.
IHC-FoFr 1/100 - 1/2000. We recommend incubating for 18-24 hours at 4C.


Anti-Mu Opioid Receptor antibody images

  • Ab10275 (1:2500) staining of Mu opioid receptor by IHC.
  • ICC/IF image of ab10275 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10275, 1 in 200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of rat brain tissue, staining Mu Opioid Receptor with ab10275.

    Tissue was blocked with 5% normal donkey serum and 0.4% Triton X-100 for 1 hour at room temperature. Sections were incubated with primary antibody (1/2000) for 24 hours at 4°C. An AlexaFluor®594-conjugated donkey anti-rabbit IgG (1/250) was used as the secondary antibody.
  • Mu Opioid Receptor (ab10275) staining of Rat DRG (dilution: 1:100) incubation at 4 °C overnight. Secondary antibody is anti-Rabbit Rhodamine Red (dilution:1:200) incubation at room temperature for 1 hour.

References for Anti-Mu Opioid Receptor antibody (ab10275)

This product has been referenced in:
  • Lantero A  et al. TGF-ß and opioid receptor signaling crosstalk results in improvement of endogenous and exogenous opioid analgesia under pathological pain conditions. J Neurosci 34:5385-95 (2014). Mouse . Read more (PubMed: 24719115) »
  • Brewer KL  et al. Dopamine D3 receptor dysfunction prevents anti-nociceptive effects of morphine in the spinal cord. Front Neural Circuits 8:62 (2014). WB ; Mouse . Read more (PubMed: 24966815) »

See all 15 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (Gross brain dissected tissue)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification Gross brain dissected tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 26 2016

Gracias por contactarnos.
Después de consultar este caso con mis compañeros del laboratorio, acerca de los siguientes anticuerpos contra Mu Opioid Receptor validados en tejidos de rata: ab10275, ab17934, ab64746, ab128013 y ab134054, me temo que n...

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Thank you for your message and for inform us about this problem.

I am sorry to hear that the free of charge replacement has not improved the results on this occasion. Due to your valuable information this antibody will now be further investiga...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for your answer.

I am sorry to confirm that we don't have any different lots in stock at the moment. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this c...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from our Anti-Mu Opioid Receptor antibody (ab10275).

I would like to reassure you that this antibody is tested and covered b...

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the origi...

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Thanks for your enquiry.

ab10275 is a whole antiserum product and the exact concentration of the anti-Mu opioid receptor in the serum has not been determined. In general, however, specific antibody concentration inproducts of this type is typ...

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Thank you for calling Abcam earlier today.

I am sorry that you have been having issues with ab10275 in IHC-Fr on mouse brain tissue sections. As we discussed on the telephone, I am sending you a new vial of the antibody (from a different lot) ...

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