Our Abpromise guarantee covers the use of ab17934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 45 kDa.


Anti-Mu Opioid Receptor antibody images

  • Predicted band size : 45 kDa

    Western Blot for ab17934

    Extracts prepared from rat brain lysates were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature and incubated with ab17934 µ-opioid receptor pan antibody (1/1000) for 2h at RT in 1% BSA-TBST buffer. After washing, the membrane was incubated with goat anti-rabbit IgG HRP conjugate. Bands were detected using the Pierce SuperSignal™ method.

References for Anti-Mu Opioid Receptor antibody (ab17934)

This product has been referenced in:
  • Niwa H  et al. Evaluation of primary opioid receptor antibodies for use in western blotting. Br J Anaesth 108:530-2 (2012). WB ; Rat . Read more (PubMed: 22337965) »

See 1 Publication for this product

Product Wall

Gracias por contactarnos.
Después de consultar este caso con mis compañeros del laboratorio, acerca de los siguientes anticuerpos contra Mu Opioid Receptor validados en tejidos de rata: ab10275, ab17934, ab64746, ab128013 y ab134054, me temo que n...

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Thank you for contacting us.
We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and this antibody (as indicated on the datasheet) has not been tested ...

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Thank you for this information. Although your samples are showing receptor expressio with another product, Abcam antibodies will have been tested under reduced and denatured conditions only and we recommend using reducing and denaturing conditions unle...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells)
Loading amount 80 µg
Specification HEK293 cells
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 01 2010

Thank you for stopping by the Abcam booth at SFN, my colleague Sarah passed your enquiry onto me. I'm sorry that you didn't receive our response back in June. Presently, ab17934 is currently in stock. The batches that we have are batches 129573, 129...

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Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire. As we understand from your e-mail, the customer used mouse Mu Opioid Receptor expressing HEK cells. We would like to emphasize that this antibody was rtaised ...

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Thank you for your enquiry. The peptide sequence is :Ac-Ala-Arg-Ile-Arg-Gln-Asn-Thr-Arg-Glu-His-Pro-Ser-Thr-Ala-Cys-NH2 and this information has been added to the datasheet. I hope this information helps, please do not hesitate to contact us if y...

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