Overview

  • Product name
  • Description
    Rabbit polyclonal to MUC13
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide from the C terminus of Human MUC13.

  • Positive control
    • Human colon tissue; 293, HeLa and COLO205 cell lysates; HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab124654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1999. Predicted molecular weight: 55 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.
  • Tissue specificity
    Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach.
  • Sequence similarities
    Contains 3 EGF-like domains.
    Contains 1 SEA domain.
  • Post-translational
    modifications
    Cleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homo-oligomerization into dimers is dependent on intrachain disulfide bonds.
    Highly N-glycosylated.
  • Cellular localization
    Cell membrane. Secreted. Also exists as a soluble form.
  • Information by UniProt
  • Database links
  • Alternative names
    • Down-regulated in colon cancer 1 antibody
    • DRCC1 antibody
    • FLJ20063 antibody
    • MUC 13 antibody
    • MUC-13 antibody
    • Muc13 antibody
    • MUC13 Down regulated in colon cancer 1 antibody
    • MUC13_HUMAN antibody
    • Mucin 13, cell surface associated antibody
    • Mucin-13 antibody
    • PRO20221 antibody
    • RECC antibody
    • UNQ6194 antibody
    see all

Images

  • ab124654 staining MUC13 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked for 30 minutes at room temperature. Samples were incubated with primary antibody (1/25 in PBS Tween) for 1 hour. An undiluted HRP polymer-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab124654, at 5 µg/ml, staining MUC13 in Formalin-fixed, Paraffin-embedded Human Colon tissue by Immunohistochemistry.
  • All lanes : Anti-MUC13 antibody (ab124654) at 1/500 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : COLO205 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HeLa cell lysate with synthesized peptide


    Predicted band size : 55 kDa
  • ab124654 at 1/100 dilution staining MUC13 in HepG2 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

References

This product has been referenced in:
  • He L  et al. Reduction of miR-132-3p contributes to gastric cancer proliferation by targeting MUC13. Mol Med Rep 15:3055-3061 (2017). WB, IHC-P ; Human . Read more (PubMed: 28339011) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Rodent Block M by Biocare Medical as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Alessandro Gandelli

Verified customer

Submitted Apr 18 2014

Thank you for your patience.

I received an answer from the laboratory. Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much informati...

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Thank you for contacting us and your interest in our products.

The immunogen sequence used to raise the anti-MUC13 antibody (ab4654) is proprietary information. However, in order to aid your research we will sometimes make an exception. The im...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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