Anti-MUC16 antibody [EPR1020(2)] (ab110640)

Overview

  • Product nameAnti-MUC16 antibody [EPR1020(2)]
    See all MUC16 primary antibodies
  • Description
    Rabbit monoclonal [EPR1020(2)] to MUC16
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MUC16.

  • Positive control
    • WB: Human uterus cell lysate. IHC-P: Human serous carcinoma and mucinous carcinoma tissues. ICC/IF: SK-OV-3 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab110640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 2353 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionThought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
    • Tissue specificityExpressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
    • Sequence similaritiesContains 2 ANK repeats.
      Contains 56 SEA domains.
    • DomainComposed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
    • Post-translational
      modifications
      Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
      Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
      May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
      May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
    • Cellular localizationCell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • CA 125 antibody
      • CA-125 antibody
      • CA125 antibody
      • CA125 ovarian cancer antigen antibody
      • Cancer antigen 125 antibody
      • FLJ14303 antibody
      • MUC 16 antibody
      • MUC-16 antibody
      • MUC16 antibody
      • MUC16_HUMAN antibody
      • Mucin 16 antibody
      • Mucin 16 cell surface associated antibody
      • Mucin-16 antibody
      • Mucin16 antibody
      • Ovarian cancer related tumor marker CA125 antibody
      • Ovarian cancer-related tumor marker CA125 antibody
      • Ovarian carcinoma antigen CA125 antibody
      see all

    Anti-MUC16 antibody [EPR1020(2)] images

    • Anti-MUC16 antibody [EPR1020(2)] (ab110640) at 1/1000 dilution + Human uterus cell lysate at 10 µg

      Predicted band size : 2353 kDa
    • Immunocytochemistry/Immunofluorescence analysis of SK-OV-3 cells labelling MUC16 with purified ab110640 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

      Control: PBS only.

    • ab110640 at 1/100 dilution staining MUC16 in Human serous carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    • ab110640 at 1/100 dilution staining MUC16 in Human mucinous carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    • ab110640 showing positive staining in Serous ovarian carcinoma tissue.

    • ab110640 showing positive staining in Normal uterus tissue.

    • ab110640 showing negative staining in Cervical carcinoma tissue.

    • ab110640 showing negative staining in Colonic adenocarcinoma tissue.

    • ab110640 showing negative staining in Normal kidney tissue.

    • ab110640 showing negative staining in Normal colon tissue.

    • ab110640 showing negative staining in Normal breast tissue.

    • ab110640 showing negative staining in Normal brain tissue.

    References for Anti-MUC16 antibody [EPR1020(2)] (ab110640)

    ab110640 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
    Sample Human Tissue sections (human endometrium)
    Specification human endometrium
    Permeabilization Yes - 0.2% Triton X-100 and PBS
    Fixative Formaldehyde
    Username

    Dr. Steffen Rickelt

    Verified customer

    Submitted Mar 17 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (human ovarian cystedenocarcinoma tissue sections)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
    Permeabilization No
    Specification human ovarian cystedenocarcinoma tissue sections
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 03 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"