Recombinant
RabMAb

Anti-MUC16 antibody [EPSISR23-96] (ab168360)

Overview

  • Product name
    Anti-MUC16 antibody [EPSISR23-96]
    See all MUC16 primary antibodies
  • Description
    Rabbit monoclonal [EPSISR23-96] to MUC16
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MUC16 aa 12450-12550.
    Database link: Q8WX17

  • Positive control
    • NIH:OVCAR-3, Human ovary cancer and Human uterus lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab168360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 1519 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
    • Tissue specificity
      Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
    • Sequence similarities
      Contains 2 ANK repeats.
      Contains 56 SEA domains.
    • Domain
      Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
    • Post-translational
      modifications
      Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
      Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
      May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
      May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
    • Cellular localization
      Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • CA 125 antibody
      • CA-125 antibody
      • CA125 antibody
      • CA125 ovarian cancer antigen antibody
      • Cancer antigen 125 antibody
      • FLJ14303 antibody
      • MUC 16 antibody
      • MUC-16 antibody
      • MUC16 antibody
      • MUC16_HUMAN antibody
      • Mucin 16 antibody
      • Mucin 16 cell surface associated antibody
      • Mucin-16 antibody
      • Mucin16 antibody
      • Ovarian cancer related tumor marker CA125 antibody
      • Ovarian cancer-related tumor marker CA125 antibody
      • Ovarian carcinoma antigen CA125 antibody
      see all

    Images

    • All lanes : Anti-MUC16 antibody [EPSISR23-96] (ab168360) at 1/1000 dilution

      Lane 1 : NIH:OVCAR-3 lysate
      Lane 2 : Human ovary cancer lysate
      Lane 3 : Human uterus lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 1519 kDa

    References

    ab168360 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab168360.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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