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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-MUC16 antibody [OC125]
See all MUC16 products (15) ...
Description
Mouse monoclonal [OC125] to MUC16
Specificity
Studies have show that this antibody reacts with approximately 80% of epithelial ovarian neoplasms of serous, endometrioid, clear cell and undifferentiated types. No reactivity has been shown for mucinous ovarian tumors or in germ cell or hematopoietic tumors. It reacts with both normal tissues and neoplasms of fallopian tube, endometrium, endocervix and mesothelioma. It does not react with colon cancer. Normal tissues such as breast, liver, skin, kidney and spleen are negative.
Tested applications
IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Partially purified human mucin fraction from a pool of tissues from patients with epithelial ovarian cancer.
Epitope
This antibody recognizes an epitope on a molecule called Cancer Antigen 125 (CA125).
Positive control
Ovarian cancer or endocervix.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: Proprietary Preservative
Constituents: 1% BSA, Tissue culture supernatant, PBS, pH 7.3
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
OC125
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MUC
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> MUC
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.(ABC Method.)
IHC-Fr: 1/50 - 1/100.(ABC Method.)
Target
Function
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Tissue specificity
Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
Sequence similarities
Contains 2 ANK repeats.
Contains 56 SEA domains.
Domain
Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
Post-translational
modifications
Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
Cellular localization
Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
Target information above from: UniProt accessionQ8WXI7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CA 125 antibody
- CA-125 antibody
- CA125 antibody
see all
Database links
- Entrez Gene: 94025 Human
- Omim: 606154 Human
- SwissProt: Q8WXI7 Human
- Unigene: 432676 Human
References for Anti-MUC16 antibody [OC125] (ab693)
This product has been referenced in:
- Kabawat SEet al. Immunopathologic characterization of a monoclonal antibody that recognizes common surface antigens of human ovarian tumors of serous, endometrioid, and clear cell types. Am J Clin Pathol 79:98-104 (1983).Read more (PubMed: 6336888) »
- Athanassiadou Pet al. Expression of cathepsin-D, CA125 and epidermal growth factor receptor in imprint smears of ovarian carcinoma. Gynecol Obstet Invest 43:125-30 (1997).Read more (PubMed: 9067721) »
See all 7 publications for this product
Publishing research using ab693? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"