Overview

  • Product nameAnti-MUC16 antibody [X325]
    See all MUC16 primary antibodies
  • Description
    Mouse monoclonal [X325] to MUC16
  • SpecificityEpitope specificity group B (ISOBM classification).
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified from human ovarian carcinoma).

  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

Applications

Our Abpromise guarantee covers the use of ab10033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IHC-P
  • Application notesELISA: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of >1000 kDa.


    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
    • Tissue specificityExpressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
    • Sequence similaritiesContains 2 ANK repeats.
      Contains 56 SEA domains.
    • DomainComposed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
    • Post-translational
      modifications
      Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
      Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
      May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
      May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
    • Cellular localizationCell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • CA 125 antibody
      • CA-125 antibody
      • CA125 antibody
      • CA125 ovarian cancer antigen antibody
      • Cancer antigen 125 antibody
      • FLJ14303 antibody
      • MUC 16 antibody
      • MUC-16 antibody
      • MUC16 antibody
      • MUC16_HUMAN antibody
      • Mucin 16 antibody
      • Mucin 16 cell surface associated antibody
      • Mucin-16 antibody
      • Mucin16 antibody
      • Ovarian cancer related tumor marker CA125 antibody
      • Ovarian cancer-related tumor marker CA125 antibody
      • Ovarian carcinoma antigen CA125 antibody
      see all

    Anti-MUC16 antibody [X325] images

    • ab10033 staining MUC16 in Human lung tissue (alveolocytes type II) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    • ab10033 staining MUC16 in Human benign ovarian tumor tissue (serous papillary cystadenoma) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    • ab10033 staining MUC16 in Human bladder carcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    References for Anti-MUC16 antibody [X325] (ab10033)

    This product has been referenced in:
    • Shimizu A  et al. Coexpression of MUC16 and mesothelin is related to the invasion process in pancreatic ductal adenocarcinoma. Cancer Sci 103:739-46 (2012). Human . Read more (PubMed: 22320398) »
    • Kesimer M  et al. Molecular organization of the mucins and glycocalyx underlying mucus transport over mucosal surfaces of the airways. Mucosal Immunol : (2012). Read more (PubMed: 22929560) »

    See all 3 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Cynomolgus Monkey Tissue sections (Tonsil)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Ventana CC1
    Permeabilization No
    Specification Tonsil
    Blocking step Dako protein block as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Fixative Formaldehyde
    Username

    Sheng Zhong

    Verified customer

    Submitted Nov 24 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated
    Sample Human Tissue sections (urinary tract)
    Specification urinary tract
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 24 2013

    Thank you for contacting us.

    Following Western blot conditions have been tested with our anti-CA-125 MAbs:

    1. CA125 is immobilized on the nitrocellulose membrane for 30 min (400 mA, 25 mM Tris-Hcl, 192 mM Glycin, 0,1% SDS, 20% ethano...

    Read More

    Thank you very much for contacting us.

    I just wanted to let you know that I am still working on your inquiry. I have contacted the originator of this product for the information you requested, and I am awaiting a reply.

    I am v...

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    Thank you for your resposne.

    As my colleauge has explained in the previous e-mail, our antibodies and proteins will regrettably not have been tested and guaranteed in the application you are using (i.e. electrical biosensors).

    As EL...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (human colon and pancreas)
    Total protein in input 5000 µg
    Specification human colon and pancreas
    Immuno-precipitation step Protein G
    Username

    Abcam user community

    Verified customer

    Submitted Aug 02 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"