Anti-MUC16 antibody [X75] (ab10029)

Overview

  • Product nameAnti-MUC16 antibody [X75]
    See all MUC16 primary antibodies
  • Description
    Mouse monoclonal [X75] to MUC16
  • SpecificityEpitope specificity group B (ISOBM classification).
  • Tested applicationsSuitable for: WB, Flow Cyt, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified from human ovarian carcinoma).

  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

Applications

Our Abpromise guarantee covers the use of ab10029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 1000 kDa.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionThought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
  • Tissue specificityExpressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
  • Sequence similaritiesContains 2 ANK repeats.
    Contains 56 SEA domains.
  • DomainComposed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
  • Post-translational
    modifications
    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
  • Cellular localizationCell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • CA 125 antibody
    • CA-125 antibody
    • CA125 antibody
    • CA125 ovarian cancer antigen antibody
    • Cancer antigen 125 antibody
    • FLJ14303 antibody
    • MUC 16 antibody
    • MUC-16 antibody
    • MUC16 antibody
    • MUC16_HUMAN antibody
    • Mucin 16 antibody
    • Mucin 16 cell surface associated antibody
    • Mucin-16 antibody
    • Mucin16 antibody
    • Ovarian cancer related tumor marker CA125 antibody
    • Ovarian cancer-related tumor marker CA125 antibody
    • Ovarian carcinoma antigen CA125 antibody
    see all

Anti-MUC16 antibody [X75] images

  • Overlay histogram showing HeLa cells stained with ab10029 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab462, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • ICC/IF image of ab10029 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10029, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MUC16 antibody [X75] (ab10029)

ab10029 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience.

The lab confirmed:

Yes, it was SDS-PAGE gel (6% or 8%) that was used for WB. A diffuse area on top of the gel around 200 kDa is detected, so it is really not going into the gel very well.

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Thank you for your resposne.

As my colleauge has explained in the previous e-mail, our antibodies and proteins will regrettably not have been tested and guaranteed in the application you are using (i.e. electrical biosensors).

As EL...

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Thank you for your email.

The gel percentage was given as 6% or 8%. With crude antigen from cells as sample,a diffuse area on top of the gel around 200 kDa is detected. Unfortunately, animage is not available.

The gel type is presu...

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Thank you for contacting us.

I have received the following information from the lab regarding your question:

Following Western blot conditions have been used with the MUC16 antibody ab10029:

1. MUC16 is immobilized on the...

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Thank you for contacting us. We offer many thousand products, it seems possible that in this particular instance we are offering the same monoclonal antibody from differing sources. Because the clone number is the same for both products, I am inclined ...

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