Overview

  • Product nameAnti-MUC16 antibody [X75]
    See all MUC16 primary antibodies
  • Description
    Mouse monoclonal [X75] to MUC16
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    CA-125 purified from human ovarian carcinoma.

  • Positive control
    • human adenocarcinoma or ovarian carcinoma cell lines or tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab1107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 24603616

Target

  • FunctionThought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
  • Tissue specificityExpressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
  • Sequence similaritiesContains 2 ANK repeats.
    Contains 56 SEA domains.
  • DomainComposed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
  • Post-translational
    modifications
    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
  • Cellular localizationCell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • CA 125 antibody
    • CA-125 antibody
    • CA125 antibody
    • CA125 ovarian cancer antigen antibody
    • Cancer antigen 125 antibody
    • FLJ14303 antibody
    • MUC 16 antibody
    • MUC-16 antibody
    • MUC16 antibody
    • MUC16_HUMAN antibody
    • Mucin 16 antibody
    • Mucin 16 cell surface associated antibody
    • Mucin-16 antibody
    • Mucin16 antibody
    • Ovarian cancer related tumor marker CA125 antibody
    • Ovarian cancer-related tumor marker CA125 antibody
    • Ovarian carcinoma antigen CA125 antibody
    see all

Anti-MUC16 antibody [X75] images

  • Overlay histogram showing HeLa cells stained with ab1107 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1107, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed HeLa cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • IHC image of ab1107 staining in human breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1107, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-MUC16 antibody [X75] (ab1107)

This product has been referenced in:
  • Vitiazeva V  et al. The O-Linked Glycome and Blood Group Antigens ABO on Mucin-Type Glycoproteins in Mucinous and Serous Epithelial Ovarian Tumors. PLoS One 10:e0130197 (2015). Read more (PubMed: 26075384) »
  • Zhang H  et al. Relationship of tumor marker CA125 and ovarian tumor stem cells: preliminary identification. J Ovarian Res 8:19 (2015). Read more (PubMed: 25886959) »

See all 7 Publications for this product

Product Wall

Thank you for contacting us.


The Isotype control for product ab1107 will be ab80201; http://www.abcam.com/Mouse-IgG1-CT6-Isotype-control-ab81216.html

I hope this information is helpful to you. Please do not hesitate to contact us...

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Thank you for contacting us. We offer many thousand products, it seems possible that in this particular instance we are offering the same monoclonal antibody from differing sources. Because the clone number is the same for both products, I am inclined ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Rat ovarian cancer)
Specification Rat ovarian cancer
Preparation Cell harvesting/tissue preparation method: Trypsinization for 4-8 minutes at 37 degrees C
Sample buffer: PBS
Fixation none
Permeabilization No
Gating Strategy Living cells by FSC vs SSC
Username

Abcam user community

Verified customer

Submitted Oct 03 2008

Hello, When the datasheets were created the clone was not added to any of them. Sorry. I don't know the reason why but none of our datasheets show the clone. Thanks, Amber Castle -----Original Message----- From: technical@abcam.com [mailt...

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Thank you for your enquiry. The antibody ab1107 has been tested in paraffin-embedded tissues of human adenocarcinoma and ovarian carcinoma. We do not know if CA-125 is trypsin-sensitive unfortunately.

Thank you for your enquiry. For ab11387, the half-life is not known. In addition I would like to refer you to the following publication for information on the molecular weight. It appears to be about 23kDa. The citation details are as follows: Berg...

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Ab1107 is an IgG1 antibody, so the molecular weight of this will be in the range of 155-165 kDa.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"