Overview

  • Product nameAnti-MUC2 antibody [996/1]
    See all MUC2 primary antibodies
  • Description
    Mouse monoclonal [996/1] to MUC2
  • Specificityab11197 recognises the human MUC2 mucin, and shows no cross-reactivity with MUC1, MUC3 or MUC4 mucins. In tissue sections ab11197 recognises colon, liver and prostate tissues strongly. The antibody recognises malignant colonic mucosa as well as normal mucosa.
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: TPTPTGTQTP TTTPITTTTT VTPTPTPTGT QTPTTTPITT T This is from the tandem repeat are of the sequence and can be found starting at amino acids 1933 and 2163

  • Positive control
    • Normal colon
  • General notes

    We have removed mouse from our list of reactive species based on contradictory results from researchers. We have had increased reports of negative staining results in IHC-P with mouse tissue. We do not batch test this antibody with mouse and we will no longer guarantee it for mouse reactivity.

     

    Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20418870
Flow Cyt Use 10µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. This product does not require protein digestion or antigen retrieval using heat treatment prior to staining of paraffin sections.
WB Use at an assay dependent concentration. Predicted molecular weight: 621.5 kDa.

Target

  • FunctionCoats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
  • Tissue specificityColon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
  • Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 2 VWFC domains.
    Contains 4 VWFD domains.
  • Post-translational
    modifications
    O-glycosylated.
    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
    At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
  • Cellular localizationSecreted. In the intestine, secreted into the inner and outer mucus layers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Intestinal mucin 2 antibody
    • Intestinal mucin-2 antibody
    • MLP antibody
    • MUC 2 antibody
    • MUC-2 antibody
    • Muc2 antibody
    • MUC2_HUMAN antibody
    • Mucin 2 antibody
    • Mucin 2 intestinal antibody
    • Mucin 2 intestinal/tracheal antibody
    • Mucin 2 oligomeric mucus/gel forming antibody
    • Mucin 2 precursor antibody
    • Mucin 2 tracheal antibody
    • Mucin like protein antibody
    • Mucin-2 antibody
    • Mucin2 antibody
    • SMUC antibody
    see all

Anti-MUC2 antibody [996/1] images

  • ab11197 staining MUC2 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with PBS + 0.2% Triton X-100 and blocked with 10% serum for 30 minutes at 37°C; antigen retrieval was by heat mediation in a citrate buffer, pH 6. Samples were incubated with primary antibody (1/300 in PBS + 3% BSA) for 2 hours at 37°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-MUC2 antibody [996/1] (ab11197)

This product has been referenced in:
  • Forbester JL  et al. Interaction of Salmonella enterica Serovar Typhimurium with Intestinal Organoids Derived from Human Induced Pluripotent Stem Cells. Infect Immun 83:2926-34 (2015). IHC . Read more (PubMed: 25964470) »
  • Luo Q  et al. Enterotoxigenic Escherichia coli secretes a highly conserved mucin-degrading metalloprotease to effectively engage intestinal epithelial cells. Infect Immun 82:509-21 (2014). Human . Read more (PubMed: 24478067) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Tumor Tissue)
Permeabilization Yes - 0.3% Triton
Specification Tumor Tissue
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Ashok kumar Dilly

Verified customer

Submitted Sep 20 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse Tissue Section (Colon))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6
Permeabilization Yes - Triton 0.2%/PBS
Specification Mouse Tissue Section (Colon)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Formaldehyde
Username

Ms. Claudia Champagne

Verified customer

Submitted Jun 22 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (peritonei tumor, Epithelial cell type)
Permeabilization No
Specification peritonei tumor, Epithelial cell type
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 07 2015

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I a...

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Thank you for contacting us.

According to our Technical FAQs, we would like to suggest to use 1 ug/ml as a starting concentration for this antibody.



Regarding the gel we would like suggest to use a gel between 5% and 7.5%. P...

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Thank you for contacting Abcam.
We have not specifically tested this antibody on immature and mature mucin. However, I have done some research. There's a paper which looks at O-glycosylation of MUC2. Specifically, this paper notices aberrant glyco...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"