• Product name
  • Description
    Rabbit polyclonal to MUC2
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic MUC2 peptide (Human) 23 amino acids long.

  • Positive control
    • Human colon carcinoma; LS 174 T colon cancer cell line.



Our Abpromise guarantee covers the use of ab76774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC: Use at an assay dependent dilution.
    IHC-P: 1/50 - 1/100. Using streptavidin-Biotin system or polymer system. High temperature antigen retrieval is recommended (boiling tissue in 10 mM citrate buffer for 10 minutes) this enhances the signal and may require further dilution of the antibody.
    IHC-Fr: 1/50 - 1/100.

    This antibody stains normal colon and colonic carcinomas where it produces intense perinuclear staining in goblet cells. It also reacts with normal and neoplastic breast tissues and with prostate carcinoma.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
    • Tissue specificity
      Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
    • Sequence similarities
      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 1 TIL (trypsin inhibitory-like) domain.
      Contains 2 VWFC domains.
      Contains 4 VWFD domains.
    • Post-translational
      May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
      At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
    • Cellular localization
      Secreted. In the intestine, secreted into the inner and outer mucus layers.
    • Information by UniProt
    • Database links
    • Alternative names
      • Intestinal mucin 2 antibody
      • Intestinal mucin-2 antibody
      • MLP antibody
      • MUC 2 antibody
      • MUC-2 antibody
      • Muc2 antibody
      • MUC2_HUMAN antibody
      • Mucin 2 antibody
      • Mucin 2 intestinal antibody
      • Mucin 2 intestinal/tracheal antibody
      • Mucin 2 oligomeric mucus/gel forming antibody
      • Mucin 2 precursor antibody
      • Mucin 2 tracheal antibody
      • Mucin like protein antibody
      • Mucin-2 antibody
      • Mucin2 antibody
      • SMUC antibody
      see all


    • ab76774 at 1/100 dilution, staining MUC2 in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). The DAB staining procedure was used with hematoxylin as counterstain.


    This product has been referenced in:
    • Xu R  et al. Effects of lactadherin on plasma D-lactic acid and small intestinal MUC2 and claudin-1 expression levels in rats with rotavirus-induced diarrhea. Exp Ther Med 11:943-950 (2016). Read more (PubMed: 26998017) »
    • Luo Q  et al. Enterotoxigenic Escherichia coli secretes a highly conserved mucin-degrading metalloprotease to effectively engage intestinal epithelial cells. Infect Immun 82:509-21 (2014). Human . Read more (PubMed: 24478067) »

    See all 4 Publications for this product

    Customer reviews and Q&As

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