Overview

  • Product nameAnti-MUC2 antibody
    See all MUC2 primary antibodies
  • Description
    Rabbit polyclonal to MUC2
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human MUC2.

  • Positive control
    • This antibody gave a positive signal in human colon tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab90007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionCoats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
  • Tissue specificityColon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
  • Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 2 VWFC domains.
    Contains 4 VWFD domains.
  • Post-translational
    modifications
    O-glycosylated.
    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
    At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
  • Cellular localizationSecreted. In the intestine, secreted into the inner and outer mucus layers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Intestinal mucin 2 antibody
    • Intestinal mucin-2 antibody
    • MLP antibody
    • MUC 2 antibody
    • MUC-2 antibody
    • Muc2 antibody
    • MUC2_HUMAN antibody
    • Mucin 2 antibody
    • Mucin 2 intestinal antibody
    • Mucin 2 intestinal/tracheal antibody
    • Mucin 2 oligomeric mucus/gel forming antibody
    • Mucin 2 precursor antibody
    • Mucin 2 tracheal antibody
    • Mucin like protein antibody
    • Mucin-2 antibody
    • Mucin2 antibody
    • SMUC antibody
    see all

Anti-MUC2 antibody images

  • IHC image of MUC2 staining in human normal colon FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90007, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • ICC/IF image of ab90007 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90007, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.

References for Anti-MUC2 antibody (ab90007)

ab90007 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Sample Human Tissue sections (human colon)
Specification human colon
Permeabilization No
Fixative Acetone
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Submitted Dec 01 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"