Anti-MUC2 antibody (ab97386)
Key features and details
- Rabbit polyclonal to MUC2
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MUC2 antibody
See all MUC2 primary antibodies -
Description
Rabbit polyclonal to MUC2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Common marmoset -
Immunogen
Synthetic peptide within Human MUC2 (C terminal). The exact sequence is proprietary. Carrier protein conjugated.
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Positive control
- IHC-P: Mouse colon tissue. Mouse and human intestine tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (6) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. -
Tissue specificity
Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 2 VWFC domains.
Contains 4 VWFD domains. -
Post-translational
modificationsO-glycosylated.
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway. -
Cellular localization
Secreted. In the intestine, secreted into the inner and outer mucus layers. - Information by UniProt
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Database links
- Entrez Gene: 4583 Human
- Entrez Gene: 17831 Mouse
- Omim: 158370 Human
- SwissProt: Q02817 Human
- SwissProt: Q80Z19 Mouse
- Unigene: 315 Human
- Unigene: 2041 Mouse
- Unigene: 461296 Mouse
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Alternative names
- Intestinal mucin 2 antibody
- Intestinal mucin-2 antibody
- MLP antibody
see all
Images
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Paraffin-embedded mouse colon tissue stained for MUC2 using ab97386 at a 1/2000 dilution.
Antigen retrieval: Citrate buffer, pH 6.0, 15 minutes.
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Paraffin-embedded mouse intestine tissue stained for MUC2 using ab97386 at a 1/2000 dilution.
Antigen retrieval: Citrate buffer, pH 6.0, 15 minutes.
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Paraffin-embedded human intestine tissue stained for MUC2 using ab97386 at a 1/750 dilution.
Antigen retrieval: Citrate buffer, pH 6.0, 15 minutes.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab97386 has been referenced in 5 publications.
- Kim KS et al. Vertical sleeve gastrectomy induces enteroendocrine cell differentiation of intestinal stem cells through bile acid signaling. JCI Insight 7:N/A (2022). PubMed: 35503251
- Guo L et al. Dietary Goji Shapes the Gut Microbiota to Prevent the Liver Injury Induced by Acute Alcohol Intake. Front Nutr 9:929776 (2022). PubMed: 35898713
- Tian M et al. Barley Leaf Insoluble Dietary Fiber Alleviated Dextran Sulfate Sodium-Induced Mice Colitis by Modulating Gut Microbiota. Nutrients 13:N/A (2021). PubMed: 33807544
- Prados ME et al. Betulinic acid hydroxamate prevents colonic inflammation and fibrosis in murine models of inflammatory bowel disease. Acta Pharmacol Sin 42:1124-1138 (2021). PubMed: 32811965
- Chiu K et al. The Impact of Di-Isononyl Phthalate Exposure on Specialized Epithelial Cells in the Colon. Toxicol Sci 184:142-153 (2021). PubMed: 34453847