Recombinant
RabMAb

Anti-MUC4 antibody [EPR9308] (ab150381)

Overview

  • Product name
    Anti-MUC4 antibody [EPR9308]
    See all MUC4 primary antibodies
  • Description
    Rabbit monoclonal [EPR9308] to MUC4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human MUC4.

  • Positive control
    • Human colon tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/750. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1:50 - 1:100.  

Target

  • Function
    May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation.
  • Tissue specificity
    Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines.
  • Sequence similarities
    Contains 1 AMOP domain.
    Contains 2 EGF-like domains.
    Contains 1 NIDO domain.
    Contains 1 VWFD domain.
  • Developmental stage
    Expressed early in the primitive gut before respiratory and digestive epithelial cells have acquired their tissue and cell specificity. Expressed at the basal surface of the epithelium from week 14 to 26 weeks and then predominantly localized in only parietal cells. Immediately before birth, found in the cytoplasm of the mucous columnar epithelial cells. In the embryo expressed in skin, then disappears late in gestation.
  • Post-translational
    modifications
    Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.
    mucrnin-4 alpha chain is highly O-glycosylated.
    mucin-4 beta chain is predominantly N-glycosylated.
  • Cellular localization
    Secreted; Cell membrane and Membrane. Secreted. Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ascites sialoglycoprotein 1 antibody
    • Ascites sialoglycoprotein 2 antibody
    • Ascites sialoglycoprotein antibody
    • ASGP antibody
    • ASGP-1 antibody
    • ASGP-2 antibody
    • HSA276359 antibody
    • MUC 4 antibody
    • MUC-4 antibody
    • Muc4 antibody
    • MUC4_HUMAN antibody
    • Mucin 4 antibody
    • Mucin 4 cell surface associated antibody
    • Mucin 4 tracheobronchial antibody
    • Mucin-4 beta chain antibody
    • Pancreatic adenocarcinoma mucin antibody
    • Testis mucin antibody
    • Tracheobronchial mucin antibody
    • Tracheobronchial mucin Fragment antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling MUC4 with Purified ab150381 at 1:750 dilution (1.44 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling MUC4 with unpurified ab150381 at a 1/50 dilution.

  • Immunohistochemical analysis of paraffin embedded Human Stomach adenocarcinoma tissue using unpurified ab150381 showing -ve staining.

  • Immunohistochemical analysis of paraffin embedded normal Human pancreas tissue using unpurified ab150381 showing -ve staining.

  • Immunohistochemical analysis of paraffin embedded Human Pancreatic carcinoma tissue using unpurified ab150381 showing +ve staining.

References

ab150381 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6, 100C, 20 minutes
Sample
Human Tissue sections (colon cancer)
Specification
colon cancer
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up