• Product nameAnti-MUC5B antibody [19.4E]
    See all MUC5B primary antibodies
  • Description
    Mouse monoclonal [19.4E] to MUC5B
  • SpecificityReacts with MUC5B. Does not react with human MUC5A or MUC5C.
  • Tested applicationsSuitable for: IHC-Fr, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Un-glycosylated, serine and threonine free synthetic peptide from the N terminal region of human MUC5B.



Our Abpromise guarantee covers the use of ab77995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 590 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Deglycosylating the tissue is critical to expose epitope.


  • FunctionGel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
  • Tissue specificityExpressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas.
  • Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 3 HAT repeats.
    Contains 3 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • DomainThe cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding.
  • Post-translational
    Highly glycosylated. C-, N- and O-glycosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2300002I04Rik antibody
    • A130042M24 antibody
    • AV085033 antibody
    • Cervical mucin antibody
    • Cervical mucin MUC5B antibody
    • High molecular weight salivary mucin MG1 antibody
    • MG1 antibody
    • MUC-5B antibody
    • MUC5 antibody
    • MUC5B antibody
    • MUC5B_HUMAN antibody
    • MUC9 antibody
    • Mucin 5 subtype B, tracheobronchial antibody
    • Mucin 5B antibody
    • Mucin 5B oligomeric mucus/gel forming antibody
    • Mucin-5 subtype B antibody
    • Mucin-5B antibody
    • Sublingual gland mucin antibody
    • tracheobronchial antibody
    see all

Anti-MUC5B antibody [19.4E] images

  • ab77995 staining MUC5B in murine lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then incubated with the primary antibody at 2µg/ml for 16 hours at 4°C. MUC5B was revealed in black and nucleus are counterstain in blue (Fastblue).
  • ab77995 staining MUC5B in murine asthma lung tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with formaldehyde, followed by incubation with the primary antibody at 4µg/ml for 16 hours at 4°C. ab77995 was coupled to biotin and Streptavidin 488 (1/250) was used to amplify the signal. Counterstained with DAPI (blue).

References for Anti-MUC5B antibody [19.4E] (ab77995)

This product has been referenced in:
  • Li Y  et al. Aberrant Mucin5B expression in lung adenocarcinomas detected by iTRAQ labeling quantitative proteomics and immunohistochemistry. Clin Proteomics 10:15 (2013). IHC-P ; Human . Read more (PubMed: 24176033) »
  • Kim D  et al. Expression of MUC1, MUC2, MUC5AC and MUC5B in Mucinous Lesions of the Breast. Pathobiology 79:144-53 (2012). IHC ; Human . Read more (PubMed: 22269464) »

See all 2 Publications for this product

Product Wall

The protocol for deglycosylation is in the paper by Cao et al.
"Mucins (MUC1 and MUC3) of gastrointestinal and breast epithelia reveal different and heterogeneous tumor-associated aberrations in glycosylation"
Cao, Y, et al (1997) J Histoc...

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I have received information from the lab regarding our anti-MUC5B antibody. The immunogen is N terminus synthetic peptide of the MUC5AC tandem repeat, no glycosylation. Exact peptide sequence is proprietary and thus unavailable. Unfortunately the pepti...

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Thank you for contacting us.

Please check the following publication for deglysosylation protocol;


I hope this information is helpful to you. Please do not hesitate to co...

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Thank you for contacting us.

I have been searching the antibodies as per your requirements. We have different anti Mucin 5AC antibodies however I am not sure which would better suits you because is not very clear from the immunogen.
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Thank you for your inquiry.

There is a protocol for deglycosylation at this website (http://www.glycotech.com/protocols/Proto3.html) and a discussion of these related to Mucin can be found in Cao, Y. et al. (1997) (http://www.ncbi.nlm.n...

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Thank you for your patience. I have received some feedback from the originator of the product. It semms that for Western Blot (and Immunohistochemistry), there is a need to perform deglycosylation in order to expose the epitope. There is a protocol...

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Thank you for contacting us, we have determined that there is no cross reactivity with MUC5A or MUC5C.  I hope this helps, please let me know if you have any additional questions or concerns.

Thank you for your call today and for your enquiry about ab77995. The dilution optimization that you mentioned on the phone sounds like a good idea to start with. I discussed the case with my colleagues, and it does sound like something in the ...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. It is important to mention and to emphasize that the human mature MUC5B protein is heavily glycosylated and it is very likely t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Asthma lung)
Specification Asthma lung
Fixative Formaldehyde
Permeabilization No

Dr. Francois Daubeuf

Verified customer

Submitted Apr 27 2011

1-10 of 11 Abreviews or Q&A