Overview

  • Product nameAnti-MUC5B antibody
    See all MUC5B primary antibodies
  • Description
    Rabbit polyclonal to MUC5B
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 5650 - 5750 of Human MUC5B.

  • Positive control
    • This antibody gave a positive signal in Human normal colon FFPE section. IF: HepG2 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab87376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionGel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
  • Tissue specificityExpressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas.
  • Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 3 HAT repeats.
    Contains 3 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • DomainThe cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding.
  • Post-translational
    modifications
    Highly glycosylated. C-, N- and O-glycosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2300002I04Rik antibody
    • A130042M24 antibody
    • AV085033 antibody
    • Cervical mucin antibody
    • Cervical mucin MUC5B antibody
    • High molecular weight salivary mucin MG1 antibody
    • MG1 antibody
    • MUC-5B antibody
    • MUC5 antibody
    • MUC5B antibody
    • MUC5B_HUMAN antibody
    • MUC9 antibody
    • Mucin 5 subtype B, tracheobronchial antibody
    • Mucin 5B antibody
    • Mucin 5B oligomeric mucus/gel forming antibody
    • Mucin-5 subtype B antibody
    • Mucin-5B antibody
    • Sublingual gland mucin antibody
    • tracheobronchial antibody
    see all

Anti-MUC5B antibody images

  • IHC image of MUC5B staining in Human normal colon FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87376, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • ICC/IF image of ab87376 stained HepG2 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87376, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MUC5B antibody (ab87376)

ab87376 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab87376.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"