Overview

  • Product nameAnti-MUC7 antibody
    See all MUC7 primary antibodies
  • Description
    Mouse monoclonal to MUC7
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: HQSPKSHFEL PHYPGLLAHQ KPFIRKSYKC LHKRCRPKLP PSPNNPPKFP NPHQPPKHPD KNSSVVNPTL VATTQIPSVT FPSASTKITT LPNVTFLPQN , corresponding to amino acids 36-136 of Human MUC7

Properties

Applications

Our Abpromise guarantee covers the use of ab55542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay function in a protective capacity by promoting the clearance of bacteria in the oral cavity and aiding in mastication, speech, and swallowing. Binds P.aeruginosa pili.
  • Tissue specificityExpressed in salivary gland tissues and only in those that contain mucous acinar cells (e.g. sublingual and submandibular glands) and not in salivary glands containing only serous acinar cells (e.g. parotid gland).
  • Involvement in diseaseGenetic variations in MUC7 are associated with susceptibility to asthma (ASTHMA) [MIM:600807]. The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.
  • Post-translational
    modifications
    N- and O-glycosylated. Contains fucose, mannose, galactose, N-acetylglucosamine and N-acetylgalactosamine.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo MG2 antibody
    • Apo-MG2 antibody
    • DKFZp686J03256 antibody
    • FLJ27047 antibody
    • MG2 antibody
    • MGC34772 antibody
    • MUC 7 antibody
    • MUC-7 antibody
    • MUC7 antibody
    • MUC7_HUMAN antibody
    • Mucin 7 antibody
    • Mucin 7, salivary antibody
    • Mucin 7, secreted antibody
    • Mucin-7 antibody
    • Salivary mucin 7 antibody
    • Salivary mucin-7 antibody
    see all

Anti-MUC7 antibody images

  • Western blot against tagged recombinant protein immunogen using ab55542 MUC7 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • ab55542 (1µg/ml) staining MUC7 in human salivary gland using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic/secreted staining of glandular epithelium. Ductal epithelium is negative.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab55542 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55542, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing MCF7 cells stained with ab55542 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55542, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-MUC7 antibody (ab55542)

This product has been referenced in:
  • Baig SM  et al. Immunoglobulin A Defines Secretions from Salivary Tissue in Post-Sistrunk Procedure Drainage. Otolaryngol Head Neck Surg : (2011). Read more (PubMed: 21493343) »
  • Finkbeiner WE  et al. Cystic fibrosis and the relationship between mucin and chloride secretion by cultures of human airway gland mucous cells. Am J Physiol Lung Cell Mol Physiol 301:L402-14 (2011). Read more (PubMed: 21724859) »

See all 3 Publications for this product

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