Overview

  • Product nameAnti-Mucin 5AC antibody [2-11M1]
    See all Mucin 5AC primary antibodies
  • Description
    Mouse monoclonal [2-11M1] to Mucin 5AC
  • Tested applicationsSuitable for: ICC, Flow Cyt, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Cat, Human, Monkey
  • Immunogen

    Mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification) Human.

  • General notes


    This antibody reacts with peptide core of gastric mucin restricted to the surface gastric epithelium. It characterised the M1-b epitope, completely destroyed by b-mercapto-ethano. The M1-b epitope is found located on a 58 kD and 40 kD peptide after trypsin treatment of native M1 mucin. This M1-b epitope coprecipites with the M1-f epitope encoded by Mucin 5AC gene. It is probably associated with the gastric mucin encoded by Mucin 5AC gene or with a gastric mucin encoded by another gastric MUC gene which polymerized with the M1/Mucin 5AC gastric mucin.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThis antibody reacts with peptide core of gastric mucin restricted to the surface gastric epithelium. It characterised the M1-b epitope, completely destroyed by b-mercapto-ethano. The M1-b epitope is found located on a 58 kD and 40 kD peptide after trypsin treatment of native M1 mucin. This M1-b epitope coprecipites with the M1-f epitope encoded by Mucin 5AC gene. It is probably associated with the gastric mucin encoded by Mucin 5AC gene or with a gastric mucin encoded by another gastric MUC gene which polymerized with the M1/Mucin 5AC gastric mucin.
  • ClonalityMonoclonal
  • Clone number2-11M1
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/1000. PubMed: 19503797
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration. The use of periodate solution (0.1 - 0.05M) for 10-20 minutes is recommended when detecting mucin by IHC.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 130 kDa.

Target

  • RelevanceMucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.
  • Cellular localizationSecreted; Cytoplasmic and cell surface
  • Database links
  • Alternative names
    • Apomucin antibody
    • Apomucin Major Airway Glycoprotein antibody
    • Gastric mucin antibody
    • LeB antibody
    • Lewis B blood group antigen antibody
    • Major Airway Glycoprotein antibody
    • MUC 5 antibody
    • MUC5 antibody
    • MUC5AC antibody
    • Mucin 5 subtype AC tracheobronchial antibody
    • Mucin 5 Subtypes A And C antibody
    • Mucin 5 subtypes A and C tracheobronchial/gastric antibody
    • Mucin 5, tracheobronchial/gastric antibody
    • Mucin 5AC antibody
    • Mucin 5AC oligomeric mucus/gel forming antibody
    • Mucin 5AC, oligomeric mucus/gel forming pseudogene antibody
    • TBM antibody
    • Tracheobronchial Mucin (TBM) antibody
    • Tracheobronchial mucin antibody
    see all

Anti-Mucin 5AC antibody [2-11M1] images

  • ab24071 at 1/1000 dilution staining MUcin 5Ac in Human bronchial epithelia by Immunocytochemistry. Well-differentiated HBE were exposed for 24 hr to mucosal PBS or mucosal SMM (Supernatant of mucopurulent material) before experiment. An immunoperoxidase detection system was used for staining.

  • Overlay histogram showing A549 cells stained with ab24071 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24071, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Mucin 5AC antibody [2-11M1] (ab24071)

This product has been referenced in:
  • Tam A  et al. Estradiol increases mucus synthesis in bronchial epithelial cells. PLoS One 9:e100633 (2014). WB, IHC-P ; Human . Read more (PubMed: 24964096) »
  • Snelgrove RJ  et al. Alternaria-derived serine protease activity drives IL-33-mediated asthma exacerbations. J Allergy Clin Immunol 134:583-592.e6 (2014). WB . Read more (PubMed: 24636086) »

See all 6 Publications for this product

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While we are unaware of any references showing this, both ab24071 and ab24072 will be guaranteed to react with cow Mucin 5AC in IHC-Fr. These antibodies are very similar and should give similar results, the only d...

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Thanks for your reply. Are these are human tracheal epithelial cells? In addition to the 1:100 antibody dilution, you should also stain your membrane with Ponceau dye to make sure that the protein transferred successfully. I look forward ...

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My colleague Heather is out of the office but I will be happy to help you further with this. I have a couple of additional questions as well. What kind of cells are you studying? Is the supernatant processed before being loaded into the gel? ...

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I am very sorry to hear the pretreatment did not resolve the problem. I understand your position and I regret not to be able to give you more specific information, I have arranged a refund on your order. Best of luck with your experiments. I hope t...

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I was informed today that pretreatment with periodate solution (0.1 - 0.05M) is recommended for 10-20 minutes when detectin mucin in IHC. There are 2 publications which describe the periodate method: Int.J. Cancer 47; 304-310 (1991) J.Immunol.M...

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