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Mucin 5AC preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(ab-) patient
Our Abpromise guarantee covers the use of ab79082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 - 2 µg/ml.
Incubate for 30 min at RT.
ab79082 staining Mucin 5AC in human lung biopsy tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was performed by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 16 hours at 4°C.
Mucin 5AC is revealed in brown (DAB impact). Nucleus (and Mucus) are counterstain in blue (Fastblue).
Fixative: Formalin 4% (Qpath), lungs were post-fixed (6h) before paraffin embedded in a tissue-tek® VIP6 automat.
Sections: 3µm on SuperFrost®.
Antigen retrieval step: Citrate buffer pH=6 (15 min at 95°C) in a water bath then slide were placed in Sequenza immunostaining with Coverplate (Shandon).
Endogenous enzyme were blocked with Dual Endogenous Enzyme Block (Dako cytomation) 15min.
Extravidin-HRP was used to increase signal (1/500, 20min, RT).