Anti-Mucin 5AC antibody [45M1] (Biotin) (ab79082)

Overview

  • Product nameAnti-Mucin 5AC antibody [45M1] (Biotin)
    See all Mucin 5AC primary antibodies
  • Description
    Mouse monoclonal [45M1] to Mucin 5AC (Biotin)
  • ConjugationBiotin
  • Specificityab79082 recognizes the peptide core of Mucin 5AC. We have data to indicate that this antibody may not cross react with Cow. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cat, Human, Pig, Monkey, Hedgehog
  • Immunogen

    Mucin 5AC preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(ab-) patient

  • EpitopeDestroyed by Beta-mercaptoethanol and proteases but not by periodate treatment.
  • Positive control
    • Rat and human stomach tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab79082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 2 µg/ml.

Incubate for 30 min at RT.

Target

  • RelevanceMucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.
  • Cellular localizationSecreted; Cytoplasmic and cell surface
  • Database links
  • Alternative names
    • Apomucin antibody
    • Apomucin Major Airway Glycoprotein antibody
    • Gastric mucin antibody
    • LeB antibody
    • Lewis B blood group antigen antibody
    • Major Airway Glycoprotein antibody
    • MUC 5 antibody
    • MUC5 antibody
    • MUC5AC antibody
    • Mucin 5 subtype AC tracheobronchial antibody
    • Mucin 5 Subtypes A And C antibody
    • Mucin 5 subtypes A and C tracheobronchial/gastric antibody
    • Mucin 5, tracheobronchial/gastric antibody
    • Mucin 5AC antibody
    • Mucin 5AC oligomeric mucus/gel forming antibody
    • Mucin 5AC, oligomeric mucus/gel forming pseudogene antibody
    • TBM antibody
    • Tracheobronchial Mucin (TBM) antibody
    • Tracheobronchial mucin antibody
    see all

Anti-Mucin 5AC antibody [45M1] (Biotin) images

  • ab79082 at 1-2µg/ml staining Mucin 5AC in human stomach by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and DAB chromogen. Note staining of extracellular mucins in the surface of the stomach. The purified form of this antibody was used for staining.
  • ab79082 at 1-2µg/ml staining Mucin 5AC in rat stomach by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and surface staining of mucous cells. The purified form of this antibody was used for staining.
  • ab79082 staining Mucin 5AC in human lung biopsy tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was performed by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 16 hours at 4°C.

     

    Mucin 5AC is revealed in brown (DAB impact). Nucleus (and Mucus) are counterstain in blue (Fastblue).

    Fixative: Formalin 4% (Qpath), lungs were post-fixed (6h) before paraffin embedded in a tissue-tek® VIP6 automat.
    Sections: 3µm on SuperFrost®.
    Antigen retrieval step: Citrate buffer pH=6 (15 min at 95°C) in a water bath then slide were placed in Sequenza immunostaining with Coverplate (Shandon).
    Endogenous enzyme were blocked with Dual Endogenous Enzyme Block (Dako cytomation) 15min.
    Extravidin-HRP was used to increase signal (1/500, 20min, RT).

    See Abreview

  • ab79082 staining Mucin 5AC in murine asthma lung tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with formaldehyde, followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. Extravidine PE (1/250) was used to amplify the signal. Mucin 5AC is visualized in pink and nucleus in blue (DAPI).

    See Abreview

References for Anti-Mucin 5AC antibody [45M1] (Biotin) (ab79082)

This product has been referenced in:
  • Jeong KI  et al. CX3CR1 Is Expressed in Differentiated Human Ciliated Airway Cells and Co-Localizes with Respiratory Syncytial Virus on Cilia in a G Protein-Dependent Manner. PLoS One 10:e0130517 (2015). IF ; Human . Read more (PubMed: 26107373) »
  • Sountoulidis A  et al. Activation of the canonical bone morphogenetic protein (BMP) pathway during lung morphogenesis and adult lung tissue repair. PLoS One 7:e41460 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22916109) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung Biopsy)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, ph=6
Permeabilization No
Specification Lung Biopsy
Fixative Paraformaldehyde
Username

Dr. Francois Daubeuf

Verified customer

Submitted Apr 28 2015

Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

Submitted Mar 05 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Sample Mouse Tissue lysate - whole (Intestin and Lung)
Specification Intestin and Lung
Treatment Tissue was mixed in Rippa buffer and denaturated in Laemmli buffer 5min ß 95¯C
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 4°C
Username

Dr. Francois Daubeuf

Verified customer

Submitted Mar 05 2015

Thank you for your reply.

To clarify, the discount I offered is not a free sample, but can be used to get a free antibody on a future order after submitting an Abreview for ab79082.

This antibody was validated using formalin-fixed p...

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Yes, Sapphire Bioscienceis our distributor in Australia.

DISCOUNT CODE: ***
Expiration date: August 28th, 2012

I am very pleased to hear you would like to accept our offer and test ab79082 in canine. This code will give you: 1 ...

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Thank you for contacting us.

Ourantibodies to Mucin 5AC that have been validated for use on frozen tissues are primarily mouse monoclonal antibodies raised against the full length protein, for which the epitopes have not been mapped. Addition...

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Thank you for contacting us.

I have been searching the antibodies as per your requirements. We have different anti Mucin 5AC antibodies however I am not sure which would better suits you because is not very clear from the immunogen.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH=6
Permeabilization No
Username

Dr. Francois Daubeuf

Verified customer

Submitted Jun 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Permeabilization No
Blocking step BSA 1%, collagen 0,5% as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

Submitted Jun 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (lung)
Specification lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 06 2011

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