Multicolor Human T cell marker panel (CD3-FITC, CD4-PE, CD8-APC and CD45-PE/Cy7®) (ab106073)

Overview

  • Product nameMulticolor Human T cell marker panel (CD3-FITC, CD4-PE, CD8-APC and CD45-PE/Cy7®)
  • Product overview

    ab106073 is a human T cell marker panel containing CD3 mouse monoclonal (FITC-conjugated), CD4 mouse monoclonal (PE-conjugated), CD8 mouse monoclonal (APC-conjugated) and CD45 mouse monoclonal (PE/Cy7-conjugated) antibodies, tested for use as a cocktail in flow cytometry.

    The human T cell marker panel contains a selection of the most popular T cell associated CD markers, conjugated to a wide spectrum of fluorochromes for optimal detection using flow cytometry. Matched isotype controls are available (ab81197, ab81200, ab91364, ab103534).

  • Tested applicationsSuitable for: Flow Cytmore details

Properties

  • Alternative names
    • CD 3
    • CD 4
    • CD 45
    • CD 8
    • CD3
    • CD4
    • CD45
    • CD8
    • flow cytometry
    • marker
    • panel
    • T cell
    • T cell panel
    • T-cell
    see all

Applications

Our Abpromise guarantee covers the use of ab106073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. Typical usage: 5-10µl of each antibody for 106 cells. Optimal volumes should be determined by the end user.

Multicolor Human T cell marker panel (CD3-FITC, CD4-PE, CD8-APC and CD45-PE/Cy7®) images

  • ab106073 staining of T cells in whole human blood by flow cytometry.

    Peripheral blood was collected into Li Heparin LH/9 ml monovettes. Red blood cells were lysed with lysis buffer (for 5 ml: 41.45 g NH4Cl, 5 g NaHCO3, 1 ml 0.5M EDTA in H2O) for 20 min at room temperature. After spinning down, the obtained cell pellet was washed twice with PBS. The cells were stained with the antibodies (5 – 10 µl of each antibody/1x106 cells in 100 µl) for 20 min at room temperature in the dark. Samples were washed twice with cell sorting buffer (3 % FCS in PBS) and resuspended. Flow cytometry was performed on a FACSCalibur (Becton Dickinson) at 488 nm (FL-1, 2, 3) and 633 nm (FL-4), and analysed using Flojo software.

    Matched isotype control antibodies were used under the same conditions ( ab81197, ab81200,

    References for Multicolor Human T cell marker panel (CD3-FITC, CD4-PE, CD8-APC and CD45-PE/Cy7®) (ab106073)

    ab106073 has not yet been referenced specifically in any publications.

Product Wall

While not an expert on PBMC populations I have been able to find the following information to help you to identify these. PBMC generally are composed of ~75% T cell lymphoma and 25% B cells, NK cells. We have a number of antibodies specific to antigen...

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I am very pleased to hear you would like to accept our offer and testab18282, ab26004, and ab69764inIHC-P.You can just place the order for the 3 antibodies in the usual way you do it (through a purchaser, online, etc.). You do not need the co...

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Thank you for your inquiry, which was passed along from my colleague.


We do have the Multicolor Human T cell marker panel, but as you know it is only tested in and guaranteed for FC.


http://www.abcam.com/Multicolor-Human...

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Thank you for your reply. I have sent an email to our rep that is in charge of orders that are shipped to , to have another vial of ab95779 sent to make up for the volume shortage of the original product. I have also asked if it is possible that this c...

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Thank you for your patience while I determined the actual volumes that you should have received. For ab95779, Anti-CD83 antibody [HB15e] (Phycoerythrin), you should have received a total volume of 2ml (2000ul), as you only received 15.ml (1500ul), I am...

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Thank you for calling Abcam earlier. I am sorry about all the shortages that you have been having recently with Abcam products. For ab26004 (part of ab106073), I have read through ****notes and it appears we are still waiting for confirmation from the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"