ab106073 is a human T cell marker panel containing CD3 mouse monoclonal (FITC-conjugated), CD4 mouse monoclonal (PE-conjugated), CD8 mouse monoclonal (APC-conjugated) and CD45 mouse monoclonal (PE/Cy7-conjugated) antibodies, tested for use as a cocktail in flow cytometry.
The human T cell marker panel contains a selection of the most popular T cell associated CD markers, conjugated to a wide spectrum of fluorochromes for optimal detection using flow cytometry. Matched isotype controls are available (ab81197, ab81200, ab91364, ab103534).
|ab18282 - Anti-CD4 antibody [MEM-241] (Phycoerythrin)||1 x 50 tests|
|ab26004 - Anti-CD8 antibody [MEM-31] (Allophycocyanin)||1 x 50 tests|
|ab46729 - Anti-CD45 antibody [F10-89-4] (PE/Cy7 ®)||1 x 50 tests|
|ab69764 - Anti-CD3 antibody [MEM-57] (FITC)||1 x 50 tests|
Our Abpromise guarantee covers the use of ab106073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. Typical usage: 5-10µl of each antibody for 106 cells. Optimal volumes should be determined by the end user.|
ab106073 staining of T cells in whole human blood by flow cytometry.
Peripheral blood was collected into Li Heparin LH/9 ml monovettes. Red blood cells were lysed with lysis buffer (for 5 ml: 41.45 g NH4Cl, 5 g NaHCO3, 1 ml 0.5M EDTA in H2O) for 20 min at room temperature. After spinning down, the obtained cell pellet was washed twice with PBS. The cells were stained with the antibodies (5 – 10 µl of each antibody/1x106 cells in 100 µl) for 20 min at room temperature in the dark. Samples were washed twice with cell sorting buffer (3 % FCS in PBS) and resuspended. Flow cytometry was performed on a FACSCalibur (Becton Dickinson) at 488 nm (FL-1, 2, 3) and 633 nm (FL-4), and analysed using Flojo software.
Matched isotype control antibodies were used under the same conditions ( ab81197, ab81200,
has not yet been referenced specifically in any publications.
References for Multicolor Human T cell marker panel (CD3-FITC, CD4-PE, CD8-APC and CD45-PE/Cy7®) (ab106073)
ab106073 has not yet been referenced specifically in any publications.