Anti-MUM1 antibody (ab104803)

Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab104803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/100.

Target

  • FunctionTranscriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
  • Tissue specificityLymphoid cells.
  • Involvement in diseaseDefects in IRF4 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving IRF4 is found in multiple myeloma. Translocation t(6;14)(p25;q32) with the IgH locus.
  • Sequence similaritiesBelongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interferon regulatory factor 4 antibody
    • IRF 4 antibody
    • IRF-4 antibody
    • Irf4 antibody
    • IRF4_HUMAN antibody
    • LSIRF antibody
    • Lymphocyte specific interferon regulatory factor antibody
    • Lymphocyte specific IRF antibody
    • Lymphocyte-specific interferon regulatory factor antibody
    • Multiple myeloma oncogene 1 antibody
    • MUM 1 antibody
    • MUM1 antibody
    • NF EM5 antibody
    • NF-EM5 antibody
    • NFEM5 antibody
    • PU.1 interaction partner antibody
    • Sfpi1/PU.1 interaction partner antibody
    • Transcriptional activator PIP antibody
    see all

Anti-MUM1 antibody images

  • Anti-MUM1 antibody (ab104803) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 52 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling MUM1 with ab104803 at 8µg/ml. Arrows indicate positively labelled squamous epithelial cells. Magnification: 400X.

References for Anti-MUM1 antibody (ab104803)

ab104803 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse pancreas tumor)
Specification mouse pancreas tumor
Fixative Methanol
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Jan 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"