Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab118808 was affinity purified using an epitope specific to MUM1 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 79 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • FunctionInvolved in the DNA damage response pathway by contributing to the maintenance of chromatin architecture. Recruited to the vicinity of DNA breaks by TP53BP1 and plays an accessory role to facilitate damage-induced chromatin changes and promoting chromatin relaxation. Required for efficient DNA repair and cell survival following DNA damage.
  • Sequence similaritiesBelongs to the MUM1 family.
    Contains 1 PWWP domain.
  • DomainThe PWWP domain mediates the interaction with nucleosomes.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Recuited to DNA damage sites via its interaction with the BRCT domain of TP53BP1.
  • Information by UniProt
  • Database links
  • Alternative names
    • EXPAND1 antibody
    • FLJ14868 antibody
    • FLJ22283 antibody
    • HSPC211 antibody
    • Melanoma associated antigen (mutated) 1 antibody
    • Melanoma ubiquitous mutated protein antibody
    • MGC131891 antibody
    • MGC163315 antibody
    • MUM 1 antibody
    • MUM-1 antibody
    • MUM1 antibody
    • MUM1_HUMAN antibody
    • Mutated melanoma associated antigen 1 antibody
    • Mutated melanoma-associated antigen 1 antibody
    • Protein expandere antibody
    • PWWP domain containing protein MUM1 antibody
    • PWWP domain-containing protein MUM1 antibody
    see all

Anti-MUM1 antibody images

  • All lanes : Anti-MUM1 antibody (ab118808) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg
    Lane 4 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 79 kDa


    Exposure time : 3 minutes
  • Detection of MUM1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118808 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-MUM1 antibody (ab118808)

ab118808 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab118808.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"