Recombinant Anti-IRF4 antibody [EPR5654(2)] (ab133298)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5654(2)] to IRF4
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IRF4 antibody [EPR5654(2)]
See all IRF4 primary antibodies -
Description
Rabbit monoclonal [EPR5654(2)] to IRF4 -
Host species
Rabbit -
Specificity
Uniprot ID - Q15306 -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IM-9 cell lysate, HuT-78 cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Dissociation constant (KD)
KD = 1.12 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5654(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
Target
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Function
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes. -
Tissue specificity
Lymphoid cells. -
Involvement in disease
Multiple myeloma -
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
Post-translational
modificationsPhosphorylation by ROCK2 regulates IL-17 and IL-21 production. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3662 Human
- SwissProt: Q15306 Human
- Unigene: 401013 Human
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Alternative names
- Interferon regulatory factor 4 antibody
- IRF 4 antibody
- IRF-4 antibody
see all
Images
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All lanes : Anti-IRF4 antibody [EPR5654(2)] (ab133298) at 1/1000 dilution
Lane 1 : IM-9 cell lysate
Lane 2 : HuT-78 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133298 has been referenced in 1 publication.
- Lou Y et al. Estradiol Suppresses TLR4-triggered Apoptosis of Decidual Stromal Cells and Drives an Anti-inflammatory TH2 Shift by Activating SGK1. Int J Biol Sci 13:434-448 (2017). PubMed: 28529452