• Product nameAnti-Munc 13-4 antibody
    See all Munc 13-4 primary antibodies
  • Description
    Goat polyclonal to Munc 13-4
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 967-980 of Human Munc 13-4 (NP_954712.1)

  • Positive control
    • Human Spleen and Human Tonsil lysates; Human Thymus tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab77057 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab77057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at an assay dependent dilution. Peptide ELISA antibody detection limit dilution: 1/128000
    IHC-P: Use at a concentration of 2.5 µg/ml.
    WB: Use at a concentration of 0.1 µg/ml. Predicted molecular weight: 123 kDa.
    Preliminary experiments gave an approx 75kDa band in Human Spleen and Human Tonsil lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 123kDa according to NP_954712.1 Other isoforms have been described in other species. The band was successfully blocked by incubation with the immunising peptide

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.
    • Tissue specificityExpressed at high levels in spleen, thymus and leukocytes. Also expressed in lung and placenta, and at very low levels in brain, heart, skeletal muscle and kidney. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
    • Involvement in diseaseDefects in UNC13D are the cause of hemophagocytic lymphohistiocytosis familial type 3 (FHL3) [MIM:608898]; also known as HPLH3. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
    • Sequence similaritiesBelongs to the unc-13 family.
      Contains 2 C2 domains.
      Contains 1 MHD1 (MUNC13 homology domain 1) domain.
      Contains 1 MHD2 (MUNC13 homology domain 2) domain.
    • DomainThe MHD1 and MHD2 domains mediate localization on recycling endosomes and lysosome.
    • Cellular localizationCytoplasm. Membrane. Late endosome. Recycling endosome. Lysosome. Colocalizes with cytotoxic granules at the plasma membrane. Localizes to endosomal exocytic vesicles.
    • Information by UniProt
    • Database links
    • FormThere are 3 isoforms produced by alternative splicing.
    • Alternative names
      • FHL 3 antibody
      • FHL3 antibody
      • FLJ00067 antibody
      • HLH 3 antibody
      • HLH3 antibody
      • HPLH 3 antibody
      • HPLH3 antibody
      • Jinx antibody
      • Munc13 4 antibody
      • Munc13-4 antibody
      • Protein unc 13 homolog D antibody
      • Protein unc-13 homolog D antibody
      • UN13D_HUMAN antibody
      • Unc 13 homolog D antibody
      • UNC 13D antibody
      • Unc-13 homolog D (C. elegans) antibody
      • Unc13 homolog D (C elegans) antibody
      • Unc13 homolog D antibody
      • UNC13, C. elegans, homolog of, D antibody
      • UNC13D antibody
      • Unc13h4 antibody
      see all

    Anti-Munc 13-4 antibody images

    • ab77057, at 2.5µg/ml, staining Munc 13-4 in paraffin-embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    References for Anti-Munc 13-4 antibody (ab77057)

    ab77057 has not yet been referenced specifically in any publications.

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