Anti-Munc 13-4 antibody [EPR4914] (ab109113)


  • Product nameAnti-Munc 13-4 antibody [EPR4914]
    See all Munc 13-4 primary antibodies
  • Description
    Rabbit monoclonal [EPR4914] to Munc 13-4
  • Tested applicationsSuitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from a region of human Munc 13-4 (Q70J99).

  • Positive control
    • A673, K562, HepG2, Molt-4, and RAW264.7.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR4914
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab109113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
ICC 1/250 - 1/500.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionPlays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.
    • Tissue specificityExpressed at high levels in spleen, thymus and leukocytes. Also expressed in lung and placenta, and at very low levels in brain, heart, skeletal muscle and kidney. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
    • Involvement in diseaseDefects in UNC13D are the cause of hemophagocytic lymphohistiocytosis familial type 3 (FHL3) [MIM:608898]; also known as HPLH3. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
    • Sequence similaritiesBelongs to the unc-13 family.
      Contains 2 C2 domains.
      Contains 1 MHD1 (MUNC13 homology domain 1) domain.
      Contains 1 MHD2 (MUNC13 homology domain 2) domain.
    • DomainThe MHD1 and MHD2 domains mediate localization on recycling endosomes and lysosome.
    • Cellular localizationCytoplasm. Membrane. Late endosome. Recycling endosome. Lysosome. Colocalizes with cytotoxic granules at the plasma membrane. Localizes to endosomal exocytic vesicles.
    • Information by UniProt
    • Database links
    • FormThere are 3 isoforms produced by alternative splicing.
    • Alternative names
      • FHL 3 antibody
      • FHL3 antibody
      • FLJ00067 antibody
      • HLH 3 antibody
      • HLH3 antibody
      • HPLH 3 antibody
      • HPLH3 antibody
      • Jinx antibody
      • Munc13 4 antibody
      • Munc13-4 antibody
      • Protein unc 13 homolog D antibody
      • Protein unc-13 homolog D antibody
      • UN13D_HUMAN antibody
      • Unc 13 homolog D antibody
      • UNC 13D antibody
      • Unc-13 homolog D (C. elegans) antibody
      • Unc13 homolog D (C elegans) antibody
      • Unc13 homolog D antibody
      • UNC13, C. elegans, homolog of, D antibody
      • UNC13D antibody
      • Unc13h4 antibody
      see all

    Anti-Munc 13-4 antibody [EPR4914] images

    • All lanes : Anti-Munc 13-4 antibody [EPR4914] (ab109113) at 1/1000 dilution

      Lane 1 : A673 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Molt-4 cell lysate
      Lane 5 : RAW264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 123 kDa
    • Flow cytometric analysis of permeabilized Molt-4 cells using ab109113 in red.

    References for Anti-Munc 13-4 antibody [EPR4914] (ab109113)

    ab109113 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us regarding Ab109113.

    The Flow Cytometry data shown on our webpage usedthe following secondary antibody from JacksonImmuno Research:

    Code Number: 111-095-144 Description:
    Fluorescein (FITC) AffiniPu...

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