Overview

  • Product nameAnti-Munc 13 antibody [Munc13-71]
    See all Munc 13 primary antibodies
  • Description
    Mouse monoclonal [Munc13-71] to Munc 13
  • Specificityab50566 recognises Munc13-1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide:

    KAKFDGAQEKFNTYVTLK

    conjugated to KLH, corresponding to amino acids 10-27 of Rat Munc 13

  • Positive control
    • Cytosolic rat brain S1 fraction

Properties

Applications

Our Abpromise guarantee covers the use of ab50566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 193 kDa.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceFunction: Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses (By similarity). Also involved in secretory granule priming in insulin secretion (By similarity). Interacts with FBXO45 (via SRY domain); leading to the degradation of UNC13A by the proteasome (By similarity). Plays a role in dendrite formation by melanocytes (PubMed:23999003). Tissue specificity: Expressed in pancreatic islet cells (PubMed:12871971). Expressed in melanocytes (PubMed:23999003). Similarity: Belongs to the unc-13 family. Contains 3 C2 domains. Contains 1 MHD1 (MUNC13 homology domain 1) domain. Contains 1 MHD2 (MUNC13 homology domain 2) domain. Contains 1 phorbol-ester/DAG-type zinc finger. Domain: The C2 domains are not involved in calcium-dependent phospholipid binding. The C-terminal region containing both MHD domains and the third C2 domain is required for synaptic vesicle priming activity.
  • Cellular localizationCytoplasm. Cell membrane; Peripheral membrane protein. Cell junction; synapse, presynaptic cell membrane. Note: Localized to the active zone of presynaptic density. Translocated to the plasma membrane as response to phorbol ester binding
  • Database links
  • Alternative names
    • KIAA1032 antibody
    • Munc13-1 antibody
    • Protein unc-13 homolog A antibody
    • UN13A antibody
    • UN13A_HUMAN antibody
    • Unc 13 homolog A antibody
    • Unc13a (gene name) antibody
    • Unc13a antibody
    • Unc13h1 (gene name) antibody
    see all

References for Anti-Munc 13 antibody [Munc13-71] (ab50566)

ab50566 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"