By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract.
Use at an assay dependent concentration. PubMed: 18487653
May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
Brain and spinal cord. Highly enriched in axons.
Involvement in disease
Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.
ICC/IF image of ab3451 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3451, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Munc18-1 antibody (ab3451)Image from Wagnon JL et al., PLoS Genet 8(11): e1003067.. Fig 6.; doi: 10.1371/journal.pgen.1003067. Epub 2012 Nov 29.
Immunohistochemical analysis of PFA-perfusion fixed mouse brain tissue, staining Munc18-1 with ab3451.
Tissue was post-fixed in 4% paraformaldehyde overnight at 4°C. Sections were incubated in a blocking buffer of 0.3% Triton-X-100, 1% BSA and 10% NGS in PBS for 2 hours at room temperature, before incubating with primary antibody (1/100) for 2 days at 4°C. An AlexaFluor®-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.
Wagnon JL et al. CELF4 regulates translation and local abundance of a vast set of mRNAs, including genes associated with regulation of synaptic function. PLoS Genet8:e1003067 (2012).
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Kim K et al. Munc18b is an essential gene in mice whose expression is limiting for secretion by airway epithelial and mast cells. Biochem J446:383-94 (2012).
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