Anti-Munc18-1 antibody [EPR4850] (ab124920)

Overview

  • Product nameAnti-Munc18-1 antibody [EPR4850]
    See all Munc18-1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4850] to Munc18-1
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Munc18.

  • Positive control
    • SH-SY5Y, HeLa, Jurkat and Y79 cell lysates; Human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionMay participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
    • Tissue specificityBrain and spinal cord. Highly enriched in axons.
    • Involvement in diseaseDefects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.
    • Sequence similaritiesBelongs to the STXBP/unc-18/SEC1 family.
    • Cellular localizationCytoplasm. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ37475 antibody
      • Munc 18 1 antibody
      • Munc 18a antibody
      • MUNC18 1 antibody
      • N-Sec1 antibody
      • Neuronal SEC1 antibody
      • NSec1 antibody
      • p67 antibody
      • Protein unc-18 homolog 1 antibody
      • Protein unc-18 homolog A antibody
      • Rb sec1 antibody
      • RBSEC1 antibody
      • STXB1_HUMAN antibody
      • STXBP1 antibody
      • Syntaxin binding protein 1 antibody
      • Syntaxin-binding protein 1 antibody
      • Unc 18 homolog antibody
      • Unc 18A antibody
      • Unc-18A antibody
      • Unc18 1 antibody
      • UNC18 antibody
      • Unc18-1 antibody
      see all

    Anti-Munc18-1 antibody [EPR4850] images

    • All lanes : Anti-Munc18-1 antibody [EPR4850] (ab124920) at 1/10000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Y79 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 68 kDa
    • ab124920, at 1/250 dilution, staining Munc18 in paraffin embedded Human brain tissue by immunohistochemistry

    References for Anti-Munc18-1 antibody [EPR4850] (ab124920)

    ab124920 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 4 µg
    Gel Running Conditions Non-reduced Denaturing
    Sample Rat Tissue lysate - whole (hippocampus tissue lysate)
    Specification hippocampus tissue lysate
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
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    Submitted Jul 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"