Munc18-1 overexpression 293T lysate (whole cell) (ab94272)

Overview

  • Product nameMunc18-1 overexpression 293T lysate (whole cell)
  • Description
    Munc18-1 overexpression lysate
  • General notesab94272 is a 293T cell transfected lysate in which Human Munc18 has been transiently over-expressed using a pCMV-Munc18 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. Tissue specificity: Brain and spinal cord. Highly enriched in axons. Disease: Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination. Similarity: Belongs to the STXBP/unc-18/SEC1 family.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Munc18-1 overexpression 293T lysate (whole cell) images

  • ab94272 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Munc18-1 antibody (ab57847) at 1/500 dilution

    Lane 1 : Munc18-1 overexpression 293T lysate (whole cell) (ab94272)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Munc18-1 overexpression 293T lysate (whole cell) (ab94272)

ab94272 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"