Anti-Munc18-1 (phospho S313) antibody (ab138687)
Key features and details
- Rabbit polyclonal to Munc18-1 (phospho S313)
- Suitable for: WB
- Reacts with: African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Munc18-1 (phospho S313) antibody
See all Munc18-1 primary antibodies -
Description
Rabbit polyclonal to Munc18-1 (phospho S313) -
Host species
Rabbit -
Specificity
ab138687 detects endogenous levels of Munc18 only when phosphorylated at S313. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: African green monkey
Predicted to work with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Munc18-1 aa 307-319 (internal sequence) (phospho S313).
Sequence:Human sequence
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Positive control
- COS7 cell extracts, treated with PMA (125ng/ml for 30mins).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 49% PBS
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab138687 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 68 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 68 kDa. |
Target
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Function
May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. -
Tissue specificity
Brain and spinal cord. Highly enriched in axons. -
Involvement in disease
Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination. -
Sequence similarities
Belongs to the STXBP/unc-18/SEC1 family. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6812 Human
- Entrez Gene: 20910 Mouse
- Entrez Gene: 25558 Rat
- Omim: 602926 Human
- SwissProt: P61764 Human
- SwissProt: O08599 Mouse
- SwissProt: P61765 Rat
- Unigene: 288229 Human
see all -
Alternative names
- FLJ37475 antibody
- Munc 18 1 antibody
- Munc 18a antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab138687 has been referenced in 1 publication.
- Simó A et al. BDNF-TrkB Signaling Coupled to nPKCe and cPKCßI Modulate the Phosphorylation of the Exocytotic Protein Munc18-1 During Synaptic Activity at the Neuromuscular Junction. Front Mol Neurosci 11:207 (2018). PubMed: 29946239