Overview

  • Product name
  • Description
    Rabbit polyclonal to MURC
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 1-90 (MEHNGSASNA DKIHQNRLSS VTEDEDQDAA LTIVTVLDKV ASIVDSVQAS QKRIEERHRE MENAIKSVQI DLLKLSQSHS NTGHIINKLF) of Human MURC.

  • Positive control
    • Human lymph node tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Induces RHOA activation and activates NPPA transcription and myofibrillar organization through the Rho/ROCK signaling pathway.
  • Sequence similarities
    Belongs to the PTRF/SDPR family.
  • Cellular localization
    Cytoplasm > myofibril > sarcomere. Cytoplasm. In cardiomyocytes, accumulates in the Z-line of the sarcomere. In vascular smooth muscle cells, detected diffusely throughout the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • murC antibody
    • MURC_HUMAN antibody
    • Muscle-related coiled-coil protein antibody
    • Muscle-restricted coiled-coil protein antibody

Anti-MURC antibody images

  • ab121644, at 1/60 dilution, staining MURC in paraffin-embedded Human lymph node tissue, by Immunohistochemistry,
  • Immunohistochemical staining of Human skeletal muscle shows distinct membranous positivity using ab121644

References for Anti-MURC antibody (ab121644)

ab121644 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Feb 15 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (5 micron thick mouse heart tissue)
Permeabilization
Yes - 0.2% Triton X-100
Specification
5 micron thick mouse heart tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 30 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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