The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionInduces RHOA activation and activates NPPA transcription and myofibrillar organization through the Rho/ROCK signaling pathway.
Sequence similaritiesBelongs to the PTRF/SDPR family.
Cellular localizationCytoplasm > myofibril > sarcomere. Cytoplasm. In cardiomyocytes, accumulates in the Z-line of the sarcomere. In vascular smooth muscle cells, detected diffusely throughout the cytoplasm.
Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and cytoskeleton (microtubules). Recommended concentration of ab121647 1-4 µg/ml. Cells treated with PFA/Triton X-100.