Overview

  • Product nameAnti-MURC antibody
    See all MURC primary antibodies
  • Description
    Rabbit polyclonal to MURC
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 95-180 (KVSAHIKDVK ARVEKQQIHV KKVEVKQEEI MKKNKFRVVI FQEKFRCPTS LSVVKDRNLT ENQEEDDDDI FDPPVDLSSD EEYYVE) of Human MURC.

  • Positive control
    • Human skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionInduces RHOA activation and activates NPPA transcription and myofibrillar organization through the Rho/ROCK signaling pathway.
  • Sequence similaritiesBelongs to the PTRF/SDPR family.
  • Cellular localizationCytoplasm > myofibril > sarcomere. Cytoplasm. In cardiomyocytes, accumulates in the Z-line of the sarcomere. In vascular smooth muscle cells, detected diffusely throughout the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • murC antibody
    • MURC_HUMAN antibody
    • Muscle-related coiled-coil protein antibody
    • Muscle-restricted coiled-coil protein antibody

Anti-MURC antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and cytoskeleton (microtubules). Recommended concentration of ab121647 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121647, at 1/1000 dilution, staining MURC in paraffin-embedded Human skeletal muscle tissue, by Immunohistochemistry.

References for Anti-MURC antibody (ab121647)

ab121647 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Heart)
Permeabilization Yes - 0.1% Triton-x 100
Specification Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Feb 16 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 10 µg
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 15 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"